Project description:Increased root H+ secretion is known as a strategy of plant adaption to low phosphorus (P) stress by enhancing mobilization of sparingly soluble P-sources. However, it remains fragmentarywhether enhanced H+ exudation could reconstruct the plant rhizosphere microbial community under low P stress. The present study found that P deficiency led to enhanced H+ exudation from soybean (Glycine max) roots. Three out of all eleven soybean H+-pyrophosphatases (GmVP) geneswere up-regulated by Pi starvation in soybean roots. Among them, GmVP2 showed the highest expression level under low P conditions. Transient expression of a GmVP2-green fluorescent protein chimera in tobacco (Nicotiana tabacum) leaves, and functional characterization of GmVP2 in transgenic soybean hairy roots demonstrated that GmVP2 encoded a plasma membrane transporter that mediated H+ exudation. Meanwhile, GmVP2-overexpression in Arabidopsis thaliana resulted in enhanced root H+ exudation, promoted plant growth, and improved sparingly soluble Ca-P utilization. Overexpression of GmVP2 also changed the rhizospheric microbial community structures, as reflected by a preferential accumulation of acidobacteria in the rhizosphere soils. These results suggested that GmVP2 mediated Pi-starvation responsive H+ exudation,which is not only involved in plant growth and mobilization of sparingly soluble P-sources, but also affects microbial community structures in soils.

2023-06-01 | GSE205273 | GEO

GeoChip data of soil microbial communities in the maize rhizosphere and bulk soils

2014-09-12 | GSE61338 | GEO

GeoChip data of soil microbial communities in the maize rhizosphere and bulk soils

Project description:Microbial communities in the rhizosphere make significant contributions to crop health and nutrient cycling. However, their ability to perform important biogeochemical processes remains uncharacterized. Important functional genes, which characterize the rhizosphere microbial community, were identified to understand metabolic capabilities in the maize rhizosphere using GeoChip 3.0-based functional gene array method. Triplicate samples were taken for both rhizosphere and bulk soil, in which each individual sample was a pool of four plants or soil cores. To determine the abundance of functional genes in the rhizosphere and bulk soils, GeoChip 3.0 was used.

2014-09-12 | E-GEOD-61338 | biostudies-arrayexpress

GeoChip data of soil microbial communities in the maize rhizosphere and bulk soils

2014-12-10 | E-MTAB-2920 | biostudies-arrayexpress

De novo RNA-sequencing and transcriptome analysis of Aconitum carmichaelii to analyze key genes involved in the biosynthesis of diterpene alkaloids

Project description:In this study, we have performed Illumina based RNA sequencing to characterize the transcriptome and expression profiles of genes expressed in 4 tissues of A. carmichaelii. RNA sequencing and de novo transcriptome assembly fo A. carmichaelii resulted in a total of 128,183 unigenes with 56,928 unigenes being annotated using NCBI-nr database. Transcriptome profile and analysis for 4 tissues of A. carmichaelii showed that unigenes annotated as possible rate-determining steps of aconitine-type biosynthetic pathway were highly expressed in the root.

2018-03-04 | GSE106247 | GEO

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