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CRISPR screens identify gene targets at breast cancer risk loci

ABSTRACT: CRISPR screens identify gene targets at breast cancer risk loci

PROVIDER: PRJNA907296 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR22483831_1.fastq.gz	Fastqsanger.gz
	SRR22483831_2.fastq.gz	Fastqsanger.gz
	SRR22483832_1.fastq.gz	Fastqsanger.gz
	SRR22483832_2.fastq.gz	Fastqsanger.gz
	SRR22483833_1.fastq.gz	Fastqsanger.gz

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CRISPR screens identify gene targets at breast cancer risk loci [HiChIP]

Project description:CRISPR screens identify gene targets at breast cancer risk loci [HiChIP]

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CRISPR screens identify gene targets at breast cancer risk loci [RNA-seq]

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CRISPR screens identify gene targets at breast cancer risk loci [ATAC-seq]

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CRISPR screens identify gene targets at breast cancer risk loci [HiChIP]

Project description:Genome-wide association studies (GWAS) have identified >200 loci associated with breast cancer (BC) risk. The majority of candidate causal variants (CCVs) are in non-coding regions and likely modulate cancer risk by regulating gene expression. However, pinpointing the exact target of the association, and identifying the phenotype it mediates, is a major challenge in the interpretation and translation of GWAS.

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CRISPR screens identify gene targets at breast cancer risk loci.

Project description:BackgroundGenome-wide association studies (GWAS) have identified > 200 loci associated with breast cancer risk. The majority of candidate causal variants are in non-coding regions and likely modulate cancer risk by regulating gene expression. However, pinpointing the exact target of the association, and identifying the phenotype it mediates, is a major challenge in the interpretation and translation of GWAS.ResultsHere, we show that pooled CRISPR screens are highly effective at identifying GWAS target genes and defining the cancer phenotypes they mediate. Following CRISPR mediated gene activation or suppression, we measure proliferation in 2D, 3D, and in immune-deficient mice, as well as the effect on DNA repair. We perform 60 CRISPR screens and identify 20 genes predicted with high confidence to be GWAS targets that promote cancer by driving proliferation or modulating the DNA damage response in breast cells. We validate the regulation of a subset of these genes by breast cancer risk variants.ConclusionsWe demonstrate that phenotypic CRISPR screens can accurately pinpoint the gene target of a risk locus. In addition to defining gene targets of risk loci associated with increased breast cancer risk, we provide a platform for identifying gene targets and phenotypes mediated by risk variants.

| S-EPMC10053147 | biostudies-literature

CRISPR screens identify gene targets at breast cancer risk loci [RNA-seq]

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CRISPR screens identify gene targets at breast cancer risk loci

Project description:This SuperSeries is composed of the SubSeries listed below.

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Minimized combinatorial CRISPR screens identify genetic interactions in autophagy

Project description:Minimized combinatorial CRISPR screens identify genetic interactions in autophagy

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CRISPR-based epigenome editing screens identify transcriptional and epigenetic regulators of human CD8 T cell function [Sorting-based CRISPR screens]

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