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The psychosis risk gene RBM12 encodes a novel repressor of GPCR/cAMP signal transduction

ABSTRACT: The psychosis risk gene RBM12 encodes a novel repressor of GPCR/cAMP signal transduction

PROVIDER: PRJNA907376 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR22956728_1.fastq.gz	Fastqsanger.gz
	SRR22956728_2.fastq.gz	Fastqsanger.gz
	SRR22956729_1.fastq.gz	Fastqsanger.gz
	SRR22956729_2.fastq.gz	Fastqsanger.gz
	SRR22956730_1.fastq.gz	Fastqsanger.gz

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The psychosis risk gene RBM12 encodes a novel repressor of GPCR/cAMP signal transduction

Project description:A deeper understanding of the regulation of G protein-coupled receptors (GPCRs) and their associated downstream cascades will provide critical insights into how these pathways shape human physiology and disease, and could yield novel therapeutic targets. Here, we validate and characterize RNA-binding motif 12 (Rbm12) as a repressor of GPCR/cAMP signaling. We established Rbm12 CRISPR KO HEK293 cells using two independent gRNAs and human iPSC-derived neurons (iNeuron) depleted of Rbm12 via CRISPR interference. Then, we performed basal gene expression profiling (HEK293) and basal + beta-2-adrenergic receptor induced (1 hour of 1 uM Isoproterenol) conditions (neurons).

2023-08-02 | GSE219195 | GEO

The psychosis risk gene RBM12 encodes a novel repressor of GPCR/cAMP signal transduction II

Project description:The psychosis risk gene RBM12 encodes a novel repressor of GPCR/cAMP signal transduction II

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The psychosis risk gene RBM12 encodes a novel repressor of GPCR/cAMP signal transduction II

Project description:To investigate beta-2-adrenergic targets in RBM12-depleted neurons derived from the parental WTc11 iPSC line

2023-08-02 | GSE229254 | GEO

The psychosis risk factor <i>RBM12</i> encodes a novel repressor of GPCR/cAMP signal transduction.

Project description:RBM12 is a high-penetrance risk factor for familial schizophrenia and psychosis, yet its precise cellular functions and the pathways to which it belongs are not known. We utilize two complementary models, HEK293 cells and human iPSC-derived neurons, and delineate RBM12 as a novel repressor of the G protein-coupled receptor/cyclic AMP/protein kinase A (GPCR/cAMP/PKA) signaling axis. We establish that loss of RBM12 leads to hyperactive cAMP production and increased PKA activity as well as altered neuronal transcriptional responses to GPCR stimulation. Notably, the cAMP and transcriptional signaling steps are subject to discrete RBM12-dependent regulation. We further demonstrate that the two RBM12 truncating variants linked to familial psychosis impact this interplay, as the mutants fail to rescue GPCR/cAMP signaling hyperactivity in cells depleted of RBM12. Lastly, we present a mechanism underlying the impaired signaling phenotypes. In agreement with its activity as an RNA-binding protein, loss of RBM12 leads to altered gene expression, including that of multiple effectors of established significance within the receptor pathway. Specifically, the abundance of adenylyl cyclases, phosphodiesterase isoforms, and PKA regulatory and catalytic subunits is impacted by RBM12 depletion. We note that these expression changes are fully consistent with the entire gamut of hyperactive signaling outputs. In summary, the current study identifies a previously unappreciated role for RBM12 in the context of the GPCR/cAMP pathway that could be explored further as a tentative molecular mechanism underlying the functions of this factor in neuronal physiology and pathophysiology.

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Subcellular location defines GPCR signal transduction

Project description:Subcellular location defines GPCR signal transduction

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Neuropsychiatric Genetics of African Populations - Psychosis (NeuroGAP-Psychosis)

Project description:Neuropsychiatric Genetics of African Populations - Psychosis (NeuroGAP-Psychosis)

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Neuropsychiatric Genetics of African Populations - Psychosis (NeuroGAP-Psychosis)

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Identification and characterizaton of RBM12 as a novel regulator of fetal hemoglobin expression [eCLIP-Seq]

Project description:The fetal-to-adult hemoglobin transition is clinically relevant as reactivation of fetal hemoglobin (HbF) significantly reduces morbidity and mortality associated with sickle cell disease (SCD) and b-thalassemia. Most studies on the developmental regulation of the globin genes, including genome-wide genetics screens, have focused on DNA binding proteins including BCL11A and ZBTB7A/LRF and their cofactors. Our understanding of RNA binding proteins (RBPs) in this process is much more limited. Two RBPs, LIN28B and IGF2BP1 are known post-transcriptional regulators of HbF production but a global view of RBPs is still lacking. Here, we carried out a CRISPR/Cas9-based screen targeting RBPs harboring RNA methyltransferase and/or RRM domains and identified RBM12 as a novel HbF suppressor. Depletion of RBM12 induced HbF expression and attenuated cell sickling in erythroid cells derived from SCD patients with minimal detrimental effects on cell maturation. Transcriptome and proteome profiling revealed that RBM12 functions independently of major known HbF regulators. Enhanced crosslinking and immunoprecipitation followed by high throughput sequencing (eCLIP-Seq) revealed strong preferential binding of RBM12 to 5’UTRs of transcripts, narrowing down the mechanism of RBM12 action. Notably, we pinpointed the first of five RRM domains as essential, and, in conjunction with a linker domain, as sufficient for RBM12-mediated HbF regulation. Our characterization of RBM12 as a negative regulator of HbF points to an additional regulatory layer of the fetal-to-adult hemoglobin switch and broadens the pool of potential therapeutic targets for SCD and b-thalassemia.

2022-06-14 | GSE200676 | GEO

Identification and characterizaton of RBM12 as a novel regulator of fetal hemoglobin expression [RNA-seq]

2022-06-14 | GSE200675 | GEO

Diurnal alterations in gene expression across striatal subregions in psychosis

Project description:Background: Psychosis is a defining feature of schizophrenia and highly prevalent in bipolar disorder. Notably, individuals suffering with these illnesses also have major disruptions in sleep and circadian rhythms, and disturbances to sleep and circadian rhythms can precipitate or exacerbate psychotic symptoms. Psychosis is associated with the striatum, though no study to date has directly measured molecular rhythms and determined how they are altered in the striatum of subjects with psychosis. Methods: Here, we perform RNA-sequencing and both differential expression and rhythmicity analyses to investigate diurnal alterations in gene expression in human postmortem striatal subregions (NAc, caudate, and putamen) in subjects with psychosis relative to unaffected comparison subjects. Results: Across regions, we find differential expression of immune-related transcripts and a substantial loss of rhythmicity in core circadian clock genes in subjects with psychosis. In the nucleus accumbens (NAc), mitochondrial-related transcripts have decreased expression in psychosis subjects, but only in those who died at night. Additionally, we find a loss of rhythmicity in small nucleolar RNAs and a gain of rhythmicity in glutamatergic signaling in the NAc of psychosis subjects. Between region comparisons indicate that rhythmicity in the caudate and putamen is far more similar in subjects with psychosis than in matched comparison subjects. Conclusions: Together, these findings reveal differential and rhythmic gene expression differences across the striatum that may contribute to striatal dysfunction and psychosis in psychotic disorders.

2022-08-31 | GSE202537 | GEO

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