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Arabidopsis thaliana

ABSTRACT: Identification of Inhibitors of Auxin Transcriptional Activation via Chemical Genetics in Arabidopsis

OTHER RELATED OMICS DATASETS IN: E-GEOD-1491

PROVIDER: PRJNA90811 | ENA |

REPOSITORIES: ENA

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Arabidopsis thaliana

Project description:Discovery of novel auxin-responsive genes of Arabidopsis using auxin inhibitors

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TMK-based cell surface auxin signaling activates cell wall acidification in Arabidopsis

Project description:The phytohormone auxin controls a myriad of processes in plants, at least in part through its regulation of cell expansion. The "acid growth hypothesis" has been proposed to explain auxin-stimulated cell expansion for five decades, but the mechanism underlying auxin-induced cell wall acidification is poorly characterized. Auxin induces the phosphorylation and activation of the plasma membrane (PM) H+-ATPase that pumps protons into the apoplast, yet how auxin activates its phosphorylation remains elusive. Here, we show that the transmembrane kinase (TMK) auxin signaling proteins interact with PM H+-ATPases and activate their phosphorylation to promote cell wall acidification and hypocotyl cell elongation in Arabidopsis. Auxin induced TMK's interaction with H+-ATPase on the plasma membrane within 1-2 minutes as well as TMK-dependent phosphorylation of the penultimate Thr residue. Genetic, biochemical, and molecular evidence demonstrates that TMKs directly phosphorylate PM H+-ATPase and are required for auxin-induced PM H+-ATPase activation, apoplastic acidification, and cell expansion. Thus, our findings reveal a crucial connection between auxin and PM H+-ATPase activation in regulating apoplastic pH changes and cell expansion via TMK-based cell surface auxin signaling.

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Effect of NJ15 on gene expression profiles in Arabidopsis thaliana seedlings

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Project description:Auxin responsive genes in the Arabidopsis stem

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Project description:Arabidopsis thaliana: auxin-treated seedlings versus control seedlings

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