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Transcriptomic analysis of glomerular endothelial cells and podocytes exposed to methylglyoxal in co-culture

ABSTRACT: Transcriptomic analysis of glomerular endothelial cells and podocytes exposed to methylglyoxal in co-culture

PROVIDER: PRJNA909259 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR22548814.fastq.gz	Fastqsanger.gz
	SRR22548815.fastq.gz	Fastqsanger.gz
	SRR22548816.fastq.gz	Fastqsanger.gz
	SRR22548817.fastq.gz	Fastqsanger.gz
	SRR22548818.fastq.gz	Fastqsanger.gz

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Transcriptomic analysis of glomerular endothelial cells and podocytes exposed to methylglyoxal in co-culture

Project description:In diabetic nephropathy (DN), glomerular endothelial cells (GECs) and podocytes undergo pathological alterations, which are influenced by metabolic changes characteristic of diabetes, including hyperglycaemia (HG) and elevated methylglyoxal (MGO) levels. However, it remains insufficiently understood what effects these metabolic factors have on GEC and podocytes and to what extent the interactions between the two cell types can modulate these effects. To address these questions, we established a co-culture system in which GECs and podocytes were grown together in close proximity, and assessed transcriptional changes in each cell type after exposure to HG and MGO. We found that HG and MGO had distinct effects on gene expression and that the effect of each treatment was markedly different between GECs and podocytes. HG treatment led to upregulation of “immediate early response” genes, particularly those of the EGR family, as well as genes involved in inflammatory responses (in GECs) or DNA replication/cell cycle (in podocytes). Interestingly, both HG and MGO led to downregulation of genes related to extracellular matrix organisation in podocytes. Crucially, the transcriptional responses of GECs and podocytes were dependent on their interaction with each other, as many of the prominently regulated genes in co-culture of the two cell types were not significantly changed when monocultures of the cells were exposed to the same stimuli. Finally, the changes in the expression of selected genes were validated in BTBR ob/ob mice, an established model of DN. This work highlights the molecular alterations in GECs and podocytes in response to the key diabetic metabolic triggers HG and MGO, as well as the central role of GEC-podocyte crosstalk in governing these responses.

2023-11-09 | GSE220226 | GEO

Transcriptomic analysis of podocytes exposed to high glucose in co-culture with glomerular endothelial cells

2023-11-09 | GSE220228 | GEO

Transcriptomic analysis of glomerular endothelial cells exposed to high glucose in co-culture with podocytes

2023-11-09 | GSE220227 | GEO

RNA-seq of podocytes treated with glomerular endothelial cell vesicles

Project description:The effect of miRNA delivery from glomerular endothelial cells (GEnCs) to podocytes in vitro was examined by mRNA epression in podocytes untreated and treated with extracellular vesicles from GEnCs under varied activation conditions.

2020-03-05 | GSE129883 | GEO

sRNA-seq of podocytes treated with glomerular endothelial cell vesicles

Project description:The effect of miRNA delivery from glomerular endothelial cells (GEnCs) to podocytes in vitro was examined by miRNA epression in podocytes untreated and treated with extracellular vesicles from GEnCs under varied activation conditions.

2020-03-05 | GSE129888 | GEO

Deep RNA Sequencing of native mouse podocytes and non-podocytes glomerular cells

Project description:Purpose: Next-generation sequencing (NGS) was used to define the transcriptome of native mouse podocytes and non-podocytes glomerular cells as part of a project aiming to define the molecular fingerprint of mouse podocytes. Method: Glomeruli from 29 Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/J x hNPHS2Cre mice at the age of 10 weeks were purified and a single cell solution was prepared to seperate GFP-expressing (podocytes) and GFP-negative (non-podocytes glomerular cells) cells by FACS sorting. RNA was extracted and prepared for further analysis using directional, polyA+ library preparation. An Illumina HiSeq2500 was used for a paired-end sequencing of 100 cycles . Salmon and Sleuth were used for downstream analysis. Results: A total of 100 Million reads each from podocytes and non-podocytes glomerular cells could be used for further analysis.

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MYDGF Deficiency Exacerbates Mitotic Catastrophe of Podocytes in Glomerular Disease

Project description:Podocytes, highly differentiated glomerular epithelial cells, are essential for the maintenance of glomerular filtration barrier. Podocyte dysfunction in podocytes is a major determinant of proteinuric kidney disease. By RNA sequencing analysis in ADR-treated podocytes with or without MYDGF overexpression, we observed the significant changes of genes important in regulating cell cycle in podocytes with ADR treatment.

2021-10-06 | GSE185226 | GEO

Transcriptomic analysis of glomerular endothelial cells exposed to high glucose in co-culture with podocytes

Project description:Transcriptomic analysis of glomerular endothelial cells exposed to high glucose in co-culture with podocytes

| PRJNA909261 | ENA

Transcriptomic analysis of podocytes exposed to high glucose in co-culture with glomerular endothelial cells

Project description:Transcriptomic analysis of podocytes exposed to high glucose in co-culture with glomerular endothelial cells

| PRJNA909260 | ENA

RNA-seq of podocytes treated with glomerular endothelial cell vesicles

Project description:RNA-seq of podocytes treated with glomerular endothelial cell vesicles

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