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SREBP modulates the NADP+/NADPH cycle to control night sleep in Drosophila

ABSTRACT: SREBP modulates the NADP+/NADPH cycle to control night sleep in Drosophila

PROVIDER: PRJNA910127 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR22575414.fastq.gz	Fastqsanger.gz
	SRR22575415.fastq.gz	Fastqsanger.gz
	SRR22575416.fastq.gz	Fastqsanger.gz
	SRR22575417.fastq.gz	Fastqsanger.gz
	SRR22575418.fastq.gz	Fastqsanger.gz

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SREBP modulates the NADP+/NADPH cycle to control night sleep in Drosophila

Project description:We investigated sleep deficits in Cyfip mutant Drosophila flies, a model for intellectual disabilities/neurodevelopmental disorders. RNA-seq was performed on heads at the circadian time point ZT16, i.e., during the night when wild-type flies are asleep.

2022-12-11 | GSE220524 | GEO

Human serum proteomics reveal molecular signature after one night of sleep deprivation

Project description:Study Objectives: Sleep deprivation is highly prevalent and caused by conditions such as night shift work or illnesses like obstructive sleep apnea. Compromised sleep is proposed to play a role in several cardiovascular, immune related and neurodegenerative disorders. We recently published human serum proteome changes after a simulated night shift. This study aimed to further explore changes in the human blood serum after 6h of sleep deprivation at night by proteomics and systems biological databases. Methods: Human blood serum samples from 8 self-declared healthy females were analyzed using mass spectrometry and high-pressure liquid chromatography. Each subject was their own control, and two samples were taken from each subject, the first one after 6h of sleep at night and the second one after 6h of sleep deprivation the following night. Biological databases and bioinformatic software were used for systems biological analyzes and comparative analysis with other published sleep-related datasets. Results: Of 494 proteins, 66 were found to be differentially expressed after 6h of sleep deprivation at night. Functional enrichment analysis revealed associations of these proteins with several biological functions related to the regulation of cellular processes like protein- and ion-binding connected to platelet degranulation and blood coagulation, as well as associations with different curated gene sets. Conclusions: This study presents serum proteomic changes after 6h of sleep deprivation, supports previous findings that only 6h of sleep deprivation affects several biological processes and revealed a molecular signature of protein changes related to pathological conditions like altered coagulation and platelet function, impaired lipid and immune function and cancer. Keywords: Human blood serum, proteomics, sleep deprivation, cellular stress, functional enrichment analysis

2024-06-27 | PXD045729 | Pride

SREBP modulates the NADP<sup>+</sup>/NADPH cycle to control night sleep in Drosophila.

Project description:Sleep behavior is conserved throughout evolution, and sleep disturbances are a frequent comorbidity of neuropsychiatric disorders. However, the molecular basis underlying sleep dysfunctions in neurological diseases remains elusive. Using a model for neurodevelopmental disorders (NDDs), the Drosophila Cytoplasmic FMR1 interacting protein haploinsufficiency (Cyfip85.1/+), we identify a mechanism modulating sleep homeostasis. We show that increased activity of the sterol regulatory element-binding protein (SREBP) in Cyfip85.1/+ flies induces an increase in the transcription of wakefulness-associated genes, such as the malic enzyme (Men), causing a disturbance in the daily NADP+/NADPH ratio oscillations and reducing sleep pressure at the night-time onset. Reduction in SREBP or Men activity in Cyfip85.1/+ flies enhances the NADP+/NADPH ratio and rescues the sleep deficits, indicating that SREBP and Men are causative for the sleep deficits in Cyfip heterozygous flies. This work suggests modulation of the SREBP metabolic axis as a new avenue worth exploring for its therapeutic potential in sleep disorders.

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HUMAN BLOOD SERUM PROTEOME CHANGES AFTER 6 HOURS OF SLEEP DEPRIVATION AT NIGHT

Project description:The aim of this study was to discover significantly changed proteins in human blood serum as a result of 6 h sleep loss at night. Eight females were reqruited. . Peripheral venous whole blood sampling was performed at 04:00, after 6 h of sleep and after 6 h of sleep deprivation (SD). Serum from each subject was depleted before protein digestion by trypsin and iTRAQ labeling. Labled peptides were analyzed by mass spectrometry.

2023-09-26 | PXD021004 | Pride

The receptor-like cytosolic kinase RIPK activates NADP-malic enzyme 2 to generate NADPH for fueling the ROS production

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Title Multi-generational mRNA sequence data of pooled Drosophila melanogaster male and female flies from populations selected for long or short night sleep

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2023-07-29 | GSE202600 | GEO

RNA-seq in acute sleep loss in humans

Project description:We investigated the genomic and physiological impact of acute sleep loss in peripheral tissues, by obtaining adipose tissue and skeletal muscle after one night of sleep loss and after one full night of sleep. Processed data (count table) only. Raw data will be submitted to EGA.

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DNA methylation in acute sleep loss in humans

Project description:We investigated the genomic and physiological impact of acute sleep loss in peripheral tissues, by obtaining adipose tissue and skeletal muscle after one night of sleep loss and after one full night of sleep. Processed data (M-values for probes not overlapping SNPs) only. Raw data will be submitted to EGA.

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The human blood transcriptome following sleep extension and sleep restriction: Heterozygote samples

Project description:In a cross balanced design human subjects were given one week of sufficient sleep and insufficient sleep (6 h per day). Following each of these conditions they were then kept awake for a day, a night and the subsequent day. During each of these periods of extended wakefulness 10 blood samples were taken, RNA was extracted from leukocytes, labelled and hybridised to human whole-genome microarrays

2017-02-08 | GSE82114 | GEO

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