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Effect of depletion of RBM4 on gene expression of esophageal squamous cell carcinoma cell KYSE150

ABSTRACT: Effect of depletion of RBM4 on gene expression of esophageal squamous cell carcinoma cell KYSE150

PROVIDER: PRJNA910527 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR22587972_1.fastq.gz	Fastqsanger.gz
	SRR22587972_2.fastq.gz	Fastqsanger.gz
	SRR22587973_1.fastq.gz	Fastqsanger.gz
	SRR22587973_2.fastq.gz	Fastqsanger.gz
	SRR22587974_1.fastq.gz	Fastqsanger.gz

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Effect of depletion of RBM4 on gene expression of esophageal squamous cell carcinoma cell KYSE150

Project description:To explore the role of RBM4 in esophageal squamous cell carcinoma cell,we performed high-throughput mRNA sequencing (mRNA-seq) in KYSE150 with stable knockdown of RBM4 by shRNA . We then performed gene expression profiling analysis using data obtained from RNA-seq of KYSE150 cells,three replicates were sequenced.

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RBM4 regulates esophageal cancer progression

Project description:In order to profile the transcription events that were regulated by RBM4, mRNA-seq was performed on KYSE150_shRBM4 and KYSE150_ctl which based on KYSE150 cells

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Next generation sequencing facilitates transcriptome analyses of mock and NCCRP1 transfected esophageal squamous cell carcinoma cell line KYSE150 cells

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The Demethylase JMJD2C/KDM4C Localizes to H3K4me3 Positive Transcription Start Sites (ChIP-seq KYSE150 cell line)

Project description:We have mapped binding sites for the histone demethylase, JMJD2C/KDM4C/GASC1, and the effect of JMJD2C depletion on H3K9me3 and H3K36me3 distributions in KYSE150 cells. The human esophageal carcinoma cell line, KYSE150, contains an amplification of the JMJD2C locus. ChIP-seq was performed using chromatin from control or JMJD2C-depleted KYSE150 cells and antibodies recognizing JMJD2C, H3K4me3, H3K9me3 or H3K36me3.

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The Demethylase JMJD2C/KDM4C Localizes to H3K4me3 Positive Transcription Start Sites (ChIP-seq KYSE150 cell line)

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Project description:We konckout PARK2 in Esophageal Squamous Cell Cancer Cell Line EC9706 and then detected differentially expressed genes We konckout PARK2 in Esophageal Squamous Cell Cancer Cell Line EC9706 and then detected differentially expressed genes

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The Demethylase JMJD2C/KDM4C Localizes to H3K4me3 Positive Transcription Start Sites (Affymetrix microarray analysis)

Project description:We have mapped transcriptional changes after depletion of the histone demethylases JMJD2C/GASC1/KDM4C and JMJD2A/KDM4A alone or in combination in the esophageal squamous carcinoma cell line, KYSE150. The KYSE150 cell line contains an amplification of the JMJD2C locus. RNA was extracted from KYSE150 cells transfected with shRNAs targeting JMJD2C and/or JMJD2A. The experiment was performed in triplicates and expression levels analyzed using Affymetrix microarrays.

2014-01-09 | E-GEOD-53892 | biostudies-arrayexpress

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