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CRISPRai Perturb-seq [Series 1]

ABSTRACT: CRISPRai Perturb-seq [Series 1]

PROVIDER: PRJNA912208 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR22741833_1.fastq.gz	Fastqsanger.gz
	SRR22741833_2.fastq.gz	Fastqsanger.gz
	SRR22741834_1.fastq.gz	Fastqsanger.gz
	SRR22741834_2.fastq.gz	Fastqsanger.gz
	SRR22741835_1.fastq.gz	Fastqsanger.gz

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CRISPRai Perturb-seq [Series 1]

Project description:We develop a system for bidirectional epigenetic editing (CRISPRai), in which orthogonal activating (CRISPRa) and repressive (CRISPRi) perturbations are applied simultaneously to multiple loci the same cell. We perform Perturb-seq, a method for single-cell RNA-seq paired with CRISPR guide RNA (gRNA) detection, on cells perturbed using CRISPRai for a panel of pairwise combinations of genes in K562 cells.

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