Project description:Cultures of three Symbiodiniaceae genera (Breviolum minutum, Durusdinium trenchii, Cladocopium sp.) were exposed to temperatures increasing from 26C to 32C over three days, followed by one week at 32C. Samples for proteome analysis were taken from controls (26C), at the end of the temperature ramp, and after one week at 32C. The three species have been submitted to PRIDE individually. Manuscript in preparation.
Project description:Cultures of three Symbiodiniaceae genera (Breviolum minutum, Durusdinium trenchii, Cladocopium sp.) were exposed to temperatures increasing from 26C to 32C over three days, followed by one week at 32C. Samples for proteome analysis were taken from controls (26C), at the end of the temperature ramp, and after one week at 32C. The three species have been submitted to PRIDE individually. Manuscript in preparation.
Project description:Cultures of three Symbiodiniaceae genera (Breviolum minutum, Durusdinium trenchii, Cladocopium sp.) were exposed to temperatures increasing from 26C to 32C over three days, followed by one week at 32C. Samples for proteome analysis were taken from controls (26C), at the end of the temperature ramp, and after one week at 32C. The three species have been submitted to PRIDE individually. Manuscript in preparation.
Project description:Dinoflagellate chromosomes represent a unique evolutionary experiment, as they exist in a permanently condensed, liquid crystalline state, are not packaged by histones, and contain genes organized into polycistronic arrays, with minimal transcriptional regulation. We analyze the 3D genome of {Breviolum minutum}, and find large topological domains without chromatin loops, demarcated by convergent gene array boundaries (``dinoTADs’’). Transcriptional inhibition degrades dinoTADs, implicating transcription-induced supercoiling as the primary topological force in dinoflagellates.
2020-07-08 | GSE153950 | GEO
Project description:Microbiome transplant from heat-evolved Cladocopium to wild-type Cladocopium
Project description:Cellular mechanisms responsible for the regulation of nutrient exchange, immune responses, and symbiont population growth in the cnidarian-dinoflagellate symbiosis are poorly resolved, particularly with respect to the dinoflagellate symbiont. Here, we characterised proteomic changes in the native symbiont Breviolum minutum during colonisation of its host sea anemone Exaiptasia diaphana (‘Aiptasia’). We also compared the proteome of this native symbiont in the established symbiotic state with that of a non-native symbiont, Durusdinium trenchii. The onset of symbiosis between Aiptasia and B. minutum induced increased accumulation of symbiont proteins associated with acquisition of inorganic carbon and photosynthesis, nitrogen metabolism, micro- and macronutrient starvation, suppression of the host immune responses, tolerance to low pH, and management of oxidative stress. Such responses are consistent with a functional, persistent symbiosis. In contrast, D. trenchii predominantly showed elevated levels of immunosuppressive proteins, consistent with the view that this symbiont is an opportunist that forms a less beneficial, less well-integrated symbiosis with this model anemone. By adding this analyses of the symbiont proteins to the already known responses of the host proteome, our results provide a more holistic view of cellular processes that determine host-symbiont specificity and how differences in symbiont partners, native versus non-native symbionts, may impact the fitness of the cnidarian-dinoflagellate symbiosis in response to thermal stress. This PRIDE entry contains the Breviolum minutum data; Durusdinium trenchii data are uploaded in a separate entry with identical parameters.