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ABSTRACT: Bacteria in lupin and alfalfa rhizosphere soil

PROVIDER: PRJNA913548 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR22801306_1.fastq.gz	Fastqsanger.gz
	SRR22801306_2.fastq.gz	Fastqsanger.gz
	SRR22801307_1.fastq.gz	Fastqsanger.gz
	SRR22801307_2.fastq.gz	Fastqsanger.gz
	SRR22801308_1.fastq.gz	Fastqsanger.gz

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Project description:fungi in lupin and alfalfa rhizosphere soil

| PRJNA913545 | ENA

Transcription profiling by array of Rhizobium leguminosarum biovar viciae strain Rlv3841grown in pea, alfalfa or sugarbeet rhizospheres compared to bacteria grown on glucose ammonia AMS medium

Project description:Rhizobium leguminosarum biovar viciae strain Rlv3841 was grown for 7 d in the pea or alfalfa or sugarbeet rhizosphere (plants all 7 d old at inoculation) and compared against bacteria grown on glucose ammonia AMS medium in the laboratory.

2010-08-04 | E-MEXP-2852 | biostudies-arrayexpress

Transcription profiling by array of Pseudomonas aeruginosa in a rhizopsphere during phosphate limitation conditions

Project description:Plants and rhizosphere microbes rely closely on each other, with plants supplying carbon to bacteria in root exudates, and bacteria mobilizing soil-bound phosphate for plant nutrition. When the phosphate supply becomes limiting for plant growth, the composition of root exudation changes, affecting rhizosphere microbial communities and microbially-mediated nutrient fluxes. To evaluate how plant phosphate deprivation affects rhizosphere bacteria, Lolium perenne seedlings were root-inoculated with Pseudomonas aeruginosa 7NR, and grown in axenic microcosms under different phosphate regimes (330 uM vs 3-6 uM phosphate). The effect of biological nutrient limitation was examined by DNA microarray studies of rhizobacterial gene expression.

2011-07-01 | E-MEXP-2812 | biostudies-arrayexpress

Project description:Alfalfa rhizosphere bacteria

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Transcription profiling by array of Rhizobium leguminosarum biovar viciae strain Rlv3841 grown rhizosphere of either pea, alfalfa or sugarbeet

Project description:Rhizobium leguminosarum biovar viciae strain Rlv3841 was grown for 7 days in the rhizosphere of either 7 day old pea, alfalfa or sugarbeet before being harvested.

2010-08-03 | E-MEXP-2849 | biostudies-arrayexpress

Niche differentiation of arsenic-transforming microbial groups in rice rhizosphere compartments as impacted by water management and soil-As concentrations

Project description:Arsenic (As) bioavailability in the rice rhizosphere is influenced by many microbial interactions, particularly by metal-transforming functional groups at the root-soil interface. This study was conducted to examine As-transforming microbes and As-speciation in the rice rhizosphere compartments, in response to two different water management practices (continuous and intermittently flooded), established on fields with high to low soil-As concentration. Microbial functional gene composition in the rhizosphere and root-plaque compartments were characterized using the GeoChip 4.0 microarray. Arsenic speciation and concentrations were analyzed in the rhizosphere soil, root-plaque, porewater and grain samples. Results indicated that intermittent flooding significantly altered As-speciation in the rhizosphere, and reduced methyl-As and AsIII concentrations in the pore water, root-plaque and rice grain. Ordination and taxonomic analysis of detected gene-probes indicated that root-plaque and rhizosphere assembled significantly different metal-transforming functional groups. Taxonomic non-redundancy was evident, suggesting that As-reduction, -oxidation and -methylation processes were performed by different microbial groups. As-transformation was coupled to different biogeochemical cycling processes establishing functional non-redundancy of rice-rhizosphere microbiome in response to both rhizosphere compartmentalization and experimental treatments. This study confirmed diverse As-biotransformation at root-soil interface and provided novel insights on their responses to water management, which can be applied for mitigating As-bioavailability and accumulation in rice grains.

2021-07-12 | GSE179671 | GEO

Project description:The rhizosphere soil bacteria of alfalfa (Medicago sativa)

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Project description:Rhizosphere is a complex system of interactions between plant roots, bacteria, fungi and animals, where the release of plant root exudates stimulates bacterial density and diversity. However, the majority of the bacteria in soil results to be unculturable but active. The aim of the present work was to characterize the microbial community associated to the root of V. vinifera cv. Pinot Noir not only under a taxonomic perspective, but also under a functional point of view, using a metaproteome approach. Our results underlined the difference between the metagenomic and metaproteomic approach and the large potentiality of proteomics in describing the environmental bacterial community and its activity. In fact, by this approach, that allows to investigate the mechanisms occurring in the rhizosphere, we showed that bacteria belonging to Streptomyces, Bacillus and Pseudomonas genera are the most active in protein expression. In the rhizosphere, the identified genera were involved mainly in phosphorus and nitrogen soil metabolism.

2022-02-28 | PXD007670 | Pride

Project description:Rhizosphere microbiota of alfalfa (bacteria)

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DNA aptamers for detection of pyoverdine pf5

Project description:The rhizosphere is a small region surrounding plant roots that is enriched in biochemicals from root exudates and populated with fungi, nematode, and bacteria. Interaction of rhizosphere organisms with plants is mainly promoted by exudates from the roots. Root exudates contain biochemicals that come from primary and secondary metabolisms of plants. These biochemicals attract microbes, which influence plant nutrition. The rhizosphere bacteria (microbiome) are vital to plant nutrient uptake and influence biotic and abiotic stress and pathogenesis. Pseudomonas is a genus of gammaproteobacteria known for its ubiquitous presence in natural habitats and its striking ecological, metabolic, and biochemical diversity. Within the genus, members of the Pseudomonas fluorescens group are common inhabitants of soil and plant surfaces, and certain strains function in the biological control of plant disease, protecting plants from infection by soilborne and aerial plant pathogens. The soil bacterium Pseudomonas protegens Pf-5 (also known as Pseudomonas fluorescens Pf-5) is a well-characterized biological strain, which is distinguished by its prolific production of the secondary metabolite, pyoverdine. Knowledge of the distribution of P. fluorescens secretory activity around plant roots is very important for understanding the interaction between P. fluorescens and plants and can be achieved by real time tracking of pyoverdine. To achieve the capability of real-time tracking in soil, we have used a structure-switching SELEX strategy to select high affinity ssDNA aptamers with specificity for pyoverdine over other siderophores. Two DNA aptamers were isolated, and their features compared. The aptamers were applied to a nanoporous aluminum oxide biosensor and demonstrated to successfully detect PYO-Pf5. This sensor provides a future opportunity to track the locations around plant roots of P. protegens and to monitor PYO-Pf5 production and movement through the soil.

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