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Parenchymal damage and myeloid inflammation link the Rag GTPase – mTORC1 axis to mammalian aging [young]

ABSTRACT: Parenchymal damage and myeloid inflammation link the Rag GTPase – mTORC1 axis to mammalian aging [young]

PROVIDER: PRJNA913832 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR22809000.fastq.gz	Fastqsanger.gz
	SRR22809001.fastq.gz	Fastqsanger.gz
	SRR22809002.fastq.gz	Fastqsanger.gz
	SRR22809003.fastq.gz	Fastqsanger.gz
	SRR22808994.fastq.gz	Fastqsanger.gz

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Parenchymal damage and myeloid inflammation link the Rag GTPase – mTORC1 axis to mammalian aging

Project description:Parenchymal damage and myeloid inflammation link the Rag GTPase – mTORC1 axis to mammalian aging

| PRJNA913829 | ENA

Parenchymal damage and myeloid inflammation link the Rag GTPase – mTORC1 axis to mammalian aging [neutrophils]

Project description:The mechanistic target of rapamycin complex 1 (mTORC1) controls cellular anabolism in response to nutrient sufficiency and growth factor signaling, and genetic and pharmacological inhibition of mTORC1 extends longevity across eukaryotes. Up to date, mouse models to analyze the mechanisms of aging governed by mTORC1 has been limited by detrimental pleiotropic consequences of genetically-increased mTORC1 activity in mice. RragcS74N/+, RragcS74C/+ and RragcT89N/+ knock-in mice endogenously express active mutant variants of RagC, a GTPase that signaling nutrient sufficiency to mTORC1. Rragcmutant/+ mice show an only moderate increase in nutrient signaling to mTORC1, and after one year of life exhibit multiple features of a premature aging phenotype that include prominent senescence in peripheral organs, parenchymal expression of inflammatory and chemo-attractant molecules, increased myeloid inflammation and extensive features of inflammaging. In vivo transplantation and ex vivo functional experiments with bone marrow-derived cells show that myeloid cells are abnormally activated by parenchymal signals evoked from Rragcmut/+ organs, to where myeloid cells extravasate to inflict additional inflammatory damage. Therapeutic suppression of myeloid inflammation in old Rragcmut/+ attenuates features of premature aging and extends longevity. We provide the first genetic link of elevated nutrient – mTORC1 activity and premature aging in mammals, and provide support for a two-component model in which increased nutrient signaling drives parenchymal damage and myeloid inflammation precipitates organ deterioration and accelerated aging.

2024-04-02 | GSE221285 | GEO

Parenchymal damage and myeloid inflammation link the Rag GTPase – mTORC1 axis to mammalian aging [young]

2024-04-02 | GSE221284 | GEO

Parenchymal damage and myeloid inflammation link the Rag GTPase – mTORC1 axis to mammalian aging [old]

2024-04-02 | GSE221283 | GEO

Parenchymal damage and myeloid inflammation link the Rag GTPase – mTORC1 axis to mammalian aging [liver]

2024-04-02 | GSE221286 | GEO

Parenchymal damage and myeloid inflammation link the Rag GTPase – mTORC1 axis to mammalian aging [old]

Project description:Parenchymal damage and myeloid inflammation link the Rag GTPase – mTORC1 axis to mammalian aging [old]

| PRJNA913833 | ENA

Parenchymal damage and myeloid inflammation link the Rag GTPase – mTORC1 axis to mammalian aging [liver]

Project description:Parenchymal damage and myeloid inflammation link the Rag GTPase – mTORC1 axis to mammalian aging [liver]

| PRJNA913834 | ENA

Parenchymal damage and myeloid inflammation link the Rag GTPase – mTORC1 axis to mammalian aging [neutrophils]

Project description:Parenchymal damage and myeloid inflammation link the Rag GTPase – mTORC1 axis to mammalian aging [neutrophils]

| PRJNA913835 | ENA

Architecture of human Rag GTPase heterodimers and their complex with mTORC1

Project description:The Rag GTPases recruit the master kinase mTORC1 to lysosomes to regulate cell growth and proliferation in response to amino acid availability. The nucleotide state of Rag heterodimers is critical for their association with mTORC1. Our cryo-EM structure of RagA/RagC in complex with mTORC1 shows the details of RagA/C binding to the RAPTOR subunit of mTORC1 and explains why only the RagAGTP/RagCGDP nucleotide state binds mTORC1. Previous kinetic studies suggested that GTP binding to one Rag locks the heterodimer to prevent GTP binding to the other. Our crystal structures and dynamics show the mechanism for this locking, and explain how oncogenic hotspot mutations disrupt this process. In contrast to allosteric activation by RHEB, Rag heterodimer binding does not change mTORC1 conformation and activates mTORC1 by targeting it to lysosomes.

2019-10-11 | PXD015778 | Pride

Parenchymal damage and myeloid inflammation link the Rag GTPase – mTORC1 axis to mammalian aging

Project description:This SuperSeries is composed of the SubSeries listed below.

2024-04-02 | GSE221287 | GEO