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Differential regulation of primary root growth during water-deficit stress in cotton

ABSTRACT: Differential regulation of primary root growth during water-deficit stress in cotton

PROVIDER: PRJNA913848 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR22810082.fastq.gz	Fastqsanger.gz
	SRR22810083.fastq.gz	Fastqsanger.gz
	SRR22810084.fastq.gz	Fastqsanger.gz
	SRR22810085.fastq.gz	Fastqsanger.gz
	SRR22810086.fastq.gz	Fastqsanger.gz

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Differential regulation of primary root growth during water-deficit stress in cotton

Project description:Cotton seedlings were subjected to water-deficit stress along with control condition for 24 hours or 48 hours and tissues emcompassing the elongation zone of primary roots were analysed for differential accumulated transcripts compared to control.

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Differential regulation of primary root growth during water-deficit stress in maize

Project description:Maize seedlings were subjected to water-deficit stress along with control condition for 24 hours or 48 hours and tissues emcompassing the elongation zone of primary roots were analysed for differential accumulated transcripts compared to control.

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Differential regulation of primary root growth during water-deficit stress

Project description:Differential regulation of primary root growth during water-deficit stress

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Differential regulation of primary root growth during water-deficit stress in maize

Project description:Differential regulation of primary root growth during water-deficit stress in maize

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Differential regulation of primary root growth during water-deficit stress

Project description:This SuperSeries is composed of the SubSeries listed below.

2023-03-01 | GSE221296 | GEO

Changes in the maize (B73) 1-cm primary root growth zone transcriptome induced by mild (-0.03 MPa) or severe water-deficit stress (-1.6 MPa)

Project description:Transcriptome sequencing (RNA-seq) was used to profile genome-wide transcript abundance in the primary root growth zone (PRGZ) of maize seedlings grown in different water deficit treatments: well-watered (-0.02 MPa), mild water deficit stress (-0.3 MPa), or severe water deficit stress (-1.6 MPa). For each water deficit treatment, the PRGZ transcriptome was profiled at 26 hours after initiation of the water deficit treatment. By comparing the abundance of each transcript under mild or severe water deficit stress relative to its abundance under well-watered conditions, we identified transcripts that are differentially regulated in the PRGZ in response to the two levels of water deficit stress.

2019-04-30 | GSE128896 | GEO

Transcriptome of soybean reproductive tissues from plants subjected to water deficit, heat stress and a combination of water deficit and heat stress.

Project description:Soybean plants were subjected to water deficit, heat stress, and combination of water deficit and heat stress. Flower parts, sepal, anther, ovary and stigma were collected from 8-10 different plants at R1 stage growing under three above mentioned stress conditions, and under control conditions 10 days after initiation of the stresses. Differential gene expression compared to control was studied using RNAseq method.

2022-12-04 | GSE218146 | GEO

Water-deficit responsive microRNAs in the primary root growth zone of maize.

Project description:BACKGROUND:MicroRNA-mediated gene regulatory networks play a significant role in plant growth and development and environmental stress responses. RESULTS:We identified 79 microRNAs (miRNAs) and multiple miRNA variants (isomiRs) belonging to 26 miRNA families in the primary root growth zone of maize seedlings grown at one of three water potentials: well-watered (- 0.02 MPa), mild water deficit stress (- 0.3 MPa), and severe water deficit stress (- 1.6 MPa). The abundances of 3 miRNAs (mild stress) and 34 miRNAs representing 17 families (severe stress) were significantly different in water-deficit stressed relative to well-watered controls (FDR < 0.05 and validated by stem loop RT-qPCR). Degradome sequencing revealed 213 miRNA-regulated transcripts and trancriptome profiling revealed that the abundance of 77 (miRNA-regulated) were regulated by water-defecit stress. miR399e,i,j-3p was strongly regulated by water-defcit stress implicating the possibility of nutrient deficiency during stress. CONCLUSIONS:We have identified a number of maize miRNAs that respond to specific water deficits applied to the primary root growth zone. We have also identified transcripts that are targets for miRNA regulation in the root growth zone under water-deficit stress. The miR399e,i,j-3p that is known to regulate phosphate uptake in response to nutrient deficiencies responds to water-deficit stress, however, at the seedling stage the seed provides adequate nutrients for root growth thus miR399e,i,j-3p may play a separate role in water-deficit responses. A water-deficit regulated maize transcript, similar to known miR399 target mimics, was identified and we hypothesized that it is another regulatory player, moderating the role of miR399e,i,j-3p, in primary root growth zone water deficit responses.

| S-EPMC6814125 | biostudies-literature

Cotton root

Project description:Cotton root Raw sequence reads

| PRJNA732449 | ENA

Daytime soybean transcriptome fluctuations during water deficit stress

Project description:By sequencing 36 cDNA libraries with Illumina technology, we identified genes differentially expressed in soybean plants in response to water deficit and genes that were either up- or down-regulated in different periods of the day. Of 54,175 predicted soybean genes (Glyma v1.1), 35.52% exhibited expression oscillations in a 24 h period. This number increased to 39.23% when plants were submitted to water deficit. Major differences in gene expression were observed in the control plants from late day (ZT16) until predawn (ZT20) periods, indicating that gene expression oscillates during the course of 24 h in normal development. Under water deficit, dissimilarity increased in all time-periods, indicating that the applied stress influenced gene expression. Results suggest that time of day, as well as light and temperature oscillations that occur considerably affect the regulation of water deficit stress response in soybean plants.

2015-07-07 | GSE69469 | GEO

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