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Effect of Transcriptional-like Effectors (TALE) on Nicotiana benthamiana leaves

ABSTRACT: Effect of Transcriptional-like Effectors (TALE) on Nicotiana benthamiana leaves

PROVIDER: PRJNA914307 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR22829615_1.fastq.gz	Fastqsanger.gz
	SRR22829615_2.fastq.gz	Fastqsanger.gz
	SRR22829616_1.fastq.gz	Fastqsanger.gz
	SRR22829616_2.fastq.gz	Fastqsanger.gz
	SRR22829617_1.fastq.gz	Fastqsanger.gz

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Effect of Transcriptional-like Effectors (TALE) on Nicotiana benthamiana leaves

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Project description:The 35S::GFP fluorescence was silenced 6-day after infiltration to Nicotiana benthamiana leaves due to the post-transcriptional gene silencing, but became stable by adding the viral suppressor 2b which repressed RNA silencing in plants. We performed the small RNA high throughput sequencing to test whether WUS can inhibit 2b functions in repressing plant RNA silencing.

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Project description:In order to find the miRNAs, target genes and signaling pathways related to the dwarf phenotype induced by NlCSP1, 35S-NlCSP1 and 35S-GFP control were infiltrated into Nicotiana benthamiana. We analyzed the differentially expressed miRNAs in the infiltrated leaves (NlCSP1_Z/GFP_Z) and the upper uninfiltrated leaves (NlCSP1_O/GFP_O) of N. benthamiana. A total of 106 and 97 differentially expressed miRNAs were identified in NlCSP1_Z/GFP_Z and NlCSP1_O/GFP_O, respectively. KEGG annotation revealed a large number of differentially expressed miRNAs involved in plant hormone signal transduction, indicating that NlCSP1 changed the hormone signal pathway in N. benthamiana.

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