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Ago2-CLASH reveals a wide repertoire of small RNA mediated regulation [CLASH-seq]

ABSTRACT: Ago2-CLASH reveals a wide repertoire of small RNA mediated regulation [CLASH-seq]

PROVIDER: PRJNA915020 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR22863835.fastq.gz	Fastqsanger.gz
	SRR22863836.fastq.gz	Fastqsanger.gz
	SRR22863837.fastq.gz	Fastqsanger.gz

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Ago2-CLASH reveals a wide repertoire of small RNA mediated regulation [CLASH-seq]

Project description:Here we present the CLASH analysis of AGO2 in HEK293 cells to address the small RNA repertoire and uncover their physiological targets. We developed an optimized bioinformatics approach of chimeric read identification to detect chimeras of high confidence. We report thousands of Ago2 target sites driven by microRNAs, but also a substantial number of Ago2 ‘drivers’ derived from fragments of other small RNAs such as tRNAs, snoRNAs, rRNAs and others. Target validation of several miRNAs delivered by 3’ Quantseq RNA-Seq.

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Ago2-CLASH reveals a wide repertoire of small RNA mediated regulation

Project description:Ago2-CLASH reveals a wide repertoire of small RNA mediated regulation

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Ago2-CLASH reveals a wide repertoire of small RNA mediated regulation [RNA-Seq]

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Ago2-CLASH reveals a wide repertoire of small RNA mediated regulation

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Mapping targets for small nucleolar RNAs in human cells [CLASH/FLASH]

Project description:We report the identification of potential novel RNA targets for box C/D snoRNAs in human HEK293 cells, using the approaches of UV crosslinking and sequencing of hybrids (CLASH), and formaldehyde crosslinking and sequencing of hybrids (FLASH).

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mRNAs associated with Ago2: Ago2, Ago2+miR-1, Ago2+miR-124, mock transfected

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