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ABSTRACT: Identification of Viruses Infecting plant samples by Nanopore sequencing

PROVIDER: PRJNA915844 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR22891751_1.fastq.gz	Fastqsanger.gz
	SRR22891752_1.fastq.gz	Fastqsanger.gz
	SRR22891753_1.fastq.gz	Fastqsanger.gz
	SRR22891754_1.fastq.gz	Fastqsanger.gz
	SRR22891755_1.fastq.gz	Fastqsanger.gz

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Analysis and comprehensive comparison of Pacbio and Nanopore-based RNA-sequencing in Arabidopsis transcriptome

Project description:Rapidly increased studies by third-generation sequencing [Pacific Biosciences (Pacbio) and Oxford Nanopore Technologies (ONT)] have been used in all kinds of research areas. Among them, the plant full-length single-molecule transcriptome studies were most used by Pacbio while ONT was rarely used. Therefore, in this study, we developed ONT RNA-sequencing methods in plants. We performed a detailed evaluation of reads from Pacbio and Nanopore PCR cDNA (ONT Pc) sequencing in plants (Arabidopsis), including the characteristics of raw data and identification of transcripts. We aimed to provide a valuable reference for applications of ONT in plant transcriptome analysis.

2019-12-15 | GSE141641 | GEO

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