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Pien-Tze-Huang Promotes Hepatocellular Carcinoma Cell Ferroptosis by Inhibiting SLC7A11-GSH-GPX4 Axis

ABSTRACT: Pien-Tze-Huang Promotes Hepatocellular Carcinoma Cell Ferroptosis by Inhibiting SLC7A11-GSH-GPX4 Axis

PROVIDER: PRJNA916217 | ENA |

REPOSITORIES: ENA

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	SRR22895715_1.fastq.gz	Fastqsanger.gz
	SRR22895715_2.fastq.gz	Fastqsanger.gz
	SRR22895716_1.fastq.gz	Fastqsanger.gz
	SRR22895716_2.fastq.gz	Fastqsanger.gz
	SRR22895717_1.fastq.gz	Fastqsanger.gz

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Similar Datasets

Pien-Tze-Huang Promotes Hepatocellular Carcinoma Cell Ferroptosis by Inhibiting SLC7A11-GSH-GPX4 Axis

Project description:We aimed to evaluate anti-cancer efficacy of Pien-Tze-Huang (PZH), and to investigate the underlying mechanisms by integrating transcriptional regulatory network analysis and experimental validation.

2022-12-31 | GSE221790 | GEO

Pien-Tze-Huang prevents hepatocellular carcinoma by inducing ferroptosis via inhibiting SLC7A11-GSH-GPX4 axis.

Project description:BackgroundMalignant transformation from hepatic fibrosis to carcinogenesis may be a therapeutic target for hepatocellular carcinoma (HCC). The aim of this study was to evaluate anti-cancer efficacy of Pien-Tze-Huang (PZH), and to investigate the underlying mechanisms by integrating transcriptional regulatory network analysis and experimental validation.MethodsA diethylnitrosamine (DEN)-induced HCC model in rats was established and used to evaluate the anti-cancer efficacy of PZH. After detecting a transcriptomic profiling, the "disease-related gene-drug effective target" interaction network was constructed, and the candidate targets of PZH against malignant transformation from hepatic fibrosis to HCC were identified and verified in vitro.ResultsPZH effectively alleviated the pathological changes of hepatic fibrosis and cirrhosis, and inhibited tumor formation and growth in DEN-induced HCC rats. Additionally, the administration of PZH reduced the levels of various hepatic function-related serological indicators significantly. Mechanically, a ferroptosis-related SLC7A11-GSH-GPX4 axis might be one of potential targets of PZH against malignant transformation from hepatic fibrosis to HCC. Especially, high SLC7A11 expression may be associated with poor prognosis of HCC patients. Experimentally, the administration of PZH markedly increased the trivalent iron and ferrous ion, suppressed the expression levels of SLC7A11 and GPX4 proteins, and reduced the GSH/GSSG ratio in the liver tissues of DEN-induced HCC rats.ConclusionsOur data offer an evidence that PZH may effectively improve the hepatic fibrosis microenvironment and prevent the occurrence of HCC through promoting ferroptosis in tumor cells via inhibiting the SLC7A11-GSH-GPX4 axis, implying that PZH may be a potential candidate drug for prevention and treatment of HCC at an early stage.

| S-EPMC10246043 | biostudies-literature

Pien Tze Huang, a traditional Chinese medicine, in maintenance treatment of metastatic colorectal cancer: a prospective, multiple center, randomized, double-blind, placebo-controlled study

Project description:Interventions: Placebo group:Placebo 0.6g bid;Pien Tze Huang group:Pien Tze Huang 0.6g bid Primary outcome(s): progression free survival Study Design: Randomized parallel controlled trial

| 2674654 | ecrin-mdr-crc

Glutathione supplementation improves fat graft survival by inhibiting ferroptosis via the SLC7A11/GPX4 axis

Project description:Background: Autologous fat grafting is hampered by unpredictable graft survival, which is potentially regulated by ferroptosis. Glutathione (GSH), a powerful antioxidant used in tissue preservation, has ferroptosis-regulating activity; however, its effects on fat grafts are unclear. This study investigated the effects and mechanisms of GSH in fat graft survival. Methods: Human lipoaspirates were transplanted subcutaneously into the backs of normal saline-treated (control) or GSH-treated nude mice. Graft survival was evaluated by magnetic resonance imaging and histology. RNA sequencing was performed to identify differentially expressed genes and enriched pathways. GSH activity was evaluated in vitro using an oxygen and glucose deprivation (OGD) model of adipose-derived stem cells. Results: Compared with control group, GSH induced better outcomes, including superior graft retention, appearance, and histological structures. RNA sequencing suggested enhanced negative regulation of ferroptosis in the GSH-treated grafts, which showed reduced lipid peroxides, better mitochondrial ultrastructure, and SLC7A11/GPX4 axis activation. In vitro, OGD-induced ferroptosis was ameliorated by GSH, which restored cell proliferation, reduced oxidative stress, and upregulated ferroptosis defense factors. Conclusions: Our study confirms that ferroptosis participates in regulating fat graft survival and that GSH exerts a protective effect by inhibiting ferroptosis. GSH-assisted lipotransfer is a promising therapeutic strategy for future clinical application.

2023-05-08 | GSE231511 | GEO

Protective effect of Pien Tze Huang on colitis-associated colorectal cancer

Project description:The goal of this study is to compare the differentially expressed genes in colon tissue of Control and AOM/DSS induced colitis-associated cancer (CAC) mouse model with or without Pien Tze Huang (PZH) treatment. The AOM/DSS induced CAC mouse model were randomly divided into 2 groups: Model and Model + PZH groups (n=5 for each group). Control mice (n=5) were set as control. mice in Control and Model groups were intragastrically with double distilled water, while mice in PZH and Model + PZH groups were intragastrically with 250 mg/kg/d of PZH for 91 days. Then the colon tissues were used to identify differentially expressed genes among different groups.

2025-01-01 | GSE230302 | GEO

Protective effect of Pien Tze Huang on colitis-associated colorectal cancer

Project description:Protective effect of Pien Tze Huang on colitis-associated colorectal cancer

| PRJNA958214 | ENA

Identification of miRNAs as biomarkers of drug response of Pien Tze Huang in Hepatic Fibrosis Treatments

Project description:Pien Tze Huang (PZH) is herbal traditional Chinese medicine which was widely utilized in Asia for hepatic diseases. We constructed two groups hepatic fibrosis mice model using CCl4, one group using PZH treatment and another group replacing PZH with double distilled water. All 12 mice were fed for 8 weeks and then killed to have their liver tissue taken out. Small RNA-seq were used to identify miRNAs of PZH medicine effect for hepatic fibrosis. We found the expression of these miRNAs (mmu-miR-205-5p, mmu-miR-3064-5p, mmu-miR-205-5p, mmu-miR-370-3p, mmu-miR-665-3p) were changed in PZH medicine treatment for hepatic fibrosis study. Furthermore, Hmga2 and Fgf9, miRNAs corresponding target genes (Sp4, Slc2a6, Tln2, Hmga2, Ank3, Pax9, Fgf9), have been reported association with hepatic fibrosis.

2021-06-28 | GSE137100 | GEO

Influence of Liver Fibrosis on Pien Tze Huang by RNA-Seq Analysis of Circular RNAs

Project description:Purpose: We investigate the regulation circRNAs in the damaged fibrotic liver treated with Pien Tze Huang (PZH) by RNA-seq. Methods: Liver tissue circRNAs expression profiles from 8-week carbon tetrachloride (CCl4)-induced, and PZH-treated and CCl4-induced liver fibrosis control mice were investigated by RNA-seq and dysregulated circRNAs in PZH-treated were identified. Various bioinformatics analyses established competing endogenous RNA (ceNRA) in circRNAs-miRNAs-mRNAs axis. Moreover, GO annotation and Kegg pathway were analyzed to identify the relational biological processes and pathway. qRT–PCR validation was performed using LX-2 cell sample pairs and SYBR Green assays. Results: We identified 91 differentially expressed circRNAs in the PZH-treated compared with control mice, of which 58 up-regulated circRNAs and 43 down-regulated circRNAs. The ceRNA network were constructed suggesting the potential role of circRNAs is the regulation of target gene expression as a miRNAs sponge. Moreover, GO and Kegg pathway analysis showed that these predicted mRNAs were related to liver fibrosis-related pathways such as TNF, PI3K-Akt, and MAPK signaling pathway. Meanwhile, the results of validation qRT-PCR of target genes in LX-2 cells were in accordance with RNA-seq with high confidence. Conclusions: We identified the dysregulated circRNAs and established functional network of circRNA-miRNA-mRNA to further reveal the potential interactions and biological processes in PZH-treated liver fibrosis mice. These findings indicate that mmu_circ_Slc25a25 may modulate the transcription of certain genes, such as Slc7a11 and Slc39a14, which may provide a more precise and comprehensive perspective for clarifying the role of PZH therapy as a potential agent for treatment of liver fibrosis.

2021-03-01 | GSE150883 | GEO

Target identification of hepatic fibrosis therapy using Pien Tze Huang based on mRNA and lncRNA

Project description:Pien Tze Huang (PZH) is a traditional Chinese medicine which was widely utilized in Asia for hepatic diseases. We construct two groups hepatic fibrosis mice model using CCl4, one group using PZH treatment and another group didn’t treatment. We found that the liver functional indices including ALB, ALT and AST were significantly increased in non-PZH treatment group. HE staining showed that inflammation was emerged surrounding the hepatic vein in the non-PZH treatment group, Masson staining revealed that there are blue collagenous fibre surrounding hepatic vein in the non-PZH treatment group, this result indicated that PZH cured the hepatic inflammation and fibrosis. We performed the mRNA and LncRNA sequencing aim to explore the drug target using PZH treatment on transcriptome level. 905 protein coding transcripts and 23 lncRNA transcripts were differentially expressed. Our research provided potential transcriptional therapeutic target for the clinical treatment using PZH.

2019-12-18 | GSE133481 | GEO

Pien Tze Huang Regulates Phosphorylation of Metabolic Enzymes in Mice of Hepatocellular Carcinoma

Project description:This study aimed to investigate the global effect of Chinese medicine formula Pien Tze Huang on hepatocellular carcinoma (HCC) compared with the anti-tumor agent sorafenib based on tandem mass tag label proteomic and phosphoproteomic analysis in addition to parallel reaction monitoring verification.

2022-10-19 | PXD037540 |

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