Project description:Bulk RNA-seq of: HUVECs co-incubated with CYR61-treated mouse bone marrow-derived macrophages CYR61-treated murine bone marrow-derived macrophages CYR61-treated HUVECs and CYR61-treated human THP1 monocytes
Project description:We assess the transcriptional changes when human umbilical vein endothelial cells are co-incubated with naïve or CYR61-activated mouse bone marrow-derived macrophages.
Project description:Unstimulated murine bone marrow derived macrophages cultured in L92 media from WT (4 biological replicates), Nrf2 KO (3 biological replicates) and Keap1 KD BMDM (3 biological replicates) were processed and analysed utilising DIA (label free) proteomic analysis. The Nrf2 KO mouse (DOI: 10.1006/bbrc.1997.6943 ) and Keap1 KD mouse ( DOI: 10.1128/MCB.01591-09) were previously published as noted.
Project description:Conditioned medium (CM) from bone marrow derived macrophages untreated or treated with LPS was collected and filtered through a 0.22-μm filter. The filtered CM was sequentially fractionated with 50-kDa and 100-kDa Amicon filters. The 50–100 kDa fraction of CM was analyzed by mass spectrometry.