Project description:To assess the effect of ERECTA on gene expression in Arabidopsis, genome wide transcript abundance was measured in Landsberg erecta (Ler) compared to Landsberg. For the differentially expressed genes, genetic variation in gene expression in the Ler/Cvi recombinant inbred line (RIL) population, resulted in the construction of a putative genetic regulatory network downstream of ERECTA.
Project description:Description: This entry contains 6 raw files. Experiment setup: Immunoaffinity purification of YFP-ERECTA from Arabidopsis thaliana. Two independent experiments conducted in 2013: first experiment has one sample and second experiment has two sample replicates. For each sample, purified YFP-ERECTA was separately digested with trypsin and chymotrypsin. LC-MS/MS analysis on a Thermo LTQ-Velos Orbitrap. MS in the orbitrap and MS/MS in the ion trap. Torii's lab provided purified YFP-ERECTA. Villen lab conducted proteomic sample preparation and MS analysis to identify phosphosites. Full search results provided (unfiltered).
raw file sample;
07090.raw YFP-ERECTA trypsin;
07091.raw YFP-ERECTA chymotrypsin;
07462.raw YFP-ERECTA trypsin;
07463.raw YFP-ERECTA chymotrypsin;
07465.raw YFP-ERECTA trypsin;
07466.raw YFP-ERECTA chymotrypsin;