Project description:Bovine granulosa cells harvested from cohort follicles at 1.3 days after wave emergence, and from dominant and subordinate follicles at 2.6 days after wave emergence. Total RNA extracted from granulosa cells of 4 cohort, 4 dominant and 4 subordinate follicles was pooled for generation of each respective SAGE library. Keywords: other
Project description:Bovine granulosa cells harvested from cohort follicles at 1.3 days after wave emergence, and from dominant and subordinate follicles at 2.6 days after wave emergence. Total RNA extracted from granulosa cells of 4 cohort, 4 dominant and 4 subordinate follicles was pooled for generation of each respective SAGE library. Keywords: other
Project description:The study aimed to uncover differential expression pattern of regulatory microRNAs in bovine granulosa cells derived from preovulatory dominant and subordinate follicles.
Project description:The expression pattern of microRNAs in granulosa cells of subordinate and dominant follicles during the early luteal phase of bovine estrous cycle
Project description:Coordinated interactions between ovarian granulosa and theca cells are required for female endocrine function and fertility. To elucidate these interactions the regulation of the granulosa and theca cell transcriptomes during bovine antral follicle development were investigated. Granulosa cells and theca cells were isolated from small (<5 mm), medium (5-10 mm), and large (>10 mm) antral bovine follicles. A microarray analysis of 24,000 bovine genes revealed that granulosa cells and theca cells each had gene sets specific to small, medium and large follicle cells. Transcripts regulated (i.e., minimally changed 1.5-fold) during antral follicle development for the granulosa cells involved 446 genes and for theca cells 248 genes. Only 28 regulated genes were common to both granulosa and theca cells. Regulated genes were functionally categorized with a focus on growth factors and cytokines expressed and regulated by the two cell types. Candidate regulatory growth factor proteins mediating both paracrine and autocrine cell-cell interactions include macrophage inflammatory protein (MIP1 beta), teratocarcinoma-derived growth factor 1 (TDGF1), stromal derived growth factor 1 (SDF1; i.e., CXCL12), growth differentiation factor 8 (GDF8), glia maturation factor gamma (GMFG), osteopontin (SPP1), angiopoietin 4 (ANGPT4), and chemokine ligands (CCL 2, 3, 5, and 8). The current study examined granulosa cell and theca cell regulated genes associated with bovine antral follicle development and identified candidate growth factors potentially involved in the regulation of cell-cell interactions required for ovarian function. Experiment Overall Design: Granulosacell RNA samples from three groups of follicles different in size - small, medium, and large (pooled untreated ovaries) are compared between each other. Each group has 2 separate biological replicas; each replica contained pooled RNA from 20-40 ovaries from 6-10 different animals.
