Project description:Sequences from the uropygial gland skin in a brood parastie - host system: great spotted cuckoos (Clamator glandarius) and Eurasian magpies (Pica pica)
Project description:The study of brood parasitism has traditionally been focused on the breeding period, but recent evidence suggests that it urgently needs a new spatio-temporal perspective to explore novel avenues on brood parasite-host co-evolutionary interactions. Many brood parasites are migrants, but their ecology outside their short breeding season is poorly known. The great spotted cuckoo (Clamator glandarius) is one of the classical models in the study of brood parasitism, however, there is very little information on its migratory strategy, route and wintering grounds. Furthermore, there is no previous information on the geographical distribution of mortality and its causes in this species; information that is critical to understand the fluctuations in cuckoo populations and detect potential conservation risks. Using satellite tracking technology, we provide novel insight into the migratory behavior and performance of the great spotted cuckoo. We found individuals from southern Spain to be long-distance nocturnal migrants that use the East Atlantic Flyway for both post and pre-breeding migration, and that winter in the western Sahel. We found evidence of individual variation in their migration route, particularly regarding their post-breeding behavior in Spain. Our study also suggests that the south of Morocco is the most dangerous area due to a large number of deaths during the post-breeding migratory period. Furthermore, we found that natural predation seems to be the main cause of death, probably due to raptors, although human activities (i.e. hunting) could also played a role in the southern Mediterranean shore. Our study offers novel findings and challenges traditional ideas on the ecology of this species providing a good example of how the new spatio-temporal perspective can expand our knowledge on brood parasites.
Project description:A Newcastle disease virus (NDV) was isolated in chicken embryonated eggs after detection by real-time reverse transcription-PCR (RRT-PCR) from a captive owl swab. The complete genome sequence of APMV-1/Rhinoptynx clamator/Brazil/22516/2009 (APMV-1, avian paramyxovirus type 1) was obtained using Illumina sequencing. Phylogenetic analysis of the complete genome classified the isolate within NDV class II genotype II.
Project description:Variation in home range size throughout the year and its causes are not well understood yet. Migratory brood parasites offer a unique opportunity to incorporate this spatio-temporal dimension into the study of the factors regulating home range dynamics. Using satellite transmitters, we tracked sixteen migratory great spotted cuckoos (Clamator glandarius) of both sexes for up to three years. We constructed home ranges in all major staging areas, from the Spanish breeding areas to the African wintering grounds, analyzed their temporal and geographical variation and investigated their main potential determinants (e.g. food and host availability). We found that home ranges were significantly larger in the breeding area compared to non-breeding areas. Using NDVI as a proxy for food availability, we showed that breeding area home ranges have significantly lower food availability per km2 than home ranges elsewhere which could explain why cuckoos use alternative areas with higher food availability before initiating migration. We also found some evidence for sex differences. Additionally, we found no indications of territoriality in this species, providing novel information into the current debate on brood parasite territoriality. Overall, food availability seems to be an important factor regulating home range dynamics and influencing migratory patterns throughout the year in great spotted cuckoos.
Project description:modENCODE_submission_5986 This submission comes from a modENCODE project of Jason Lieb. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. Our 126 strategically selected targets include RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents. We will integrate information generated with existing knowledge on the biology of the targets and perform ChIP-seq analysis on mutant and RNAi extracts lacking selected target proteins. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf EXPERIMENT TYPE: CHIP-seq. BIOLOGICAL SOURCE: Strain: N2; Developmental Stage: L3 Larva; Genotype: wild type; Sex: mixed Male and Hermaphrodite population; EXPERIMENTAL FACTORS: Developmental Stage L3 Larva; temp (temperature) 20 degree celsius; Strain N2; Antibody NURF-1 SDQ3525 (target is NURF-1)