Project description:AtR8 is a long non-coding RNA transcribed by RNA polymerase III in Arabidopsis which is responsive to hypoxic stress and salicylic acid. To understand the role of AtR8 lncRNA, microarray was carried out in the mutant seedlings of AtR8 and compared with wild type seedlings.
Project description:Plants often face combinatorial stresses in their natural environment. Here arsenic (As) toxicity was combined with hypoxia (Hpx) in the roots of Arabidopsis thaliana as it often occurs in nature. The present work aimed to explore the effects of single and combined hypoxia and As stress applied at realistic stress levels to hydroponically grown A. thaliana. Arsenic as well as hypoxic growth conditions generate a characteristic signaling pattern, a significant part of which is mediated by ROS. The current study utilized the microarray to determine the overlapping signalling pattern and changes in gene expression for the defined stress combinaton compared to individual stresses.
