Project description:E. coli K-12 ATCC 25404 in LB medium with 5-fluorouracil 10 uM biofilm cells relative to E. coli K-12 ATCC 25404 in LB DMF biofilm cells. The same amount of stock 5-fluoroacil stock solution (0.1% of the volume) was added as DMF into the LB DMF.
Project description:E. coli K-12 ATCC 25404 in LB medium with 5-fluorouracil 10 uM biofilm cells relative to E. coli K-12 ATCC 25404 in LB DMF biofilm cells. The same amount of stock 5-fluoroacil stock solution (0.1% of the volume) was added as DMF into the LB DMF. Experiment Overall Design: Strain: E. coli K-12 ATCC 25404 wild-type Experiment Overall Design: Medium: LB Experiment Overall Design: Biofilm growth on glass wool Experiment Overall Design: Time: 24 h Experiment Overall Design: Cell type: biofilm Experiment Overall Design: 5-fluorouracil vs. DMF