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ABSTRACT:
PROVIDER: PRJNA922240 | ENA |
REPOSITORIES: ENA
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Project description:The transcription regulator ACTR controls ACT-toxin biosynthesis and pathogenicity in the tangerine pathotype of Alternaria alternata
| PRJNA686827 | ENA
Project description:AAL toxin (the major virulence factor of Alternaria alternata f. sp. lycopersici)was treated to wild type(Col-0)and transgenic Arabidopsis with enhanced GSH (AtECS1). A proteomic profile was obtained by nano LC-MS/MS analysis.Among the identified proteins, a few proteins were selected for qRT-PCR and western blot analysis. It was found that AAL mediated resistance in plants can be conferred by GSH through SA and ET mediated pathways, in addition to several stress responsive molecules.
2020-08-07 | PXD017124 | Pride
Project description:To understand the transcriptome changes of source-sink transition leaves after infection of Alternaria alternata at 1 dpi
2014-12-01 | GSE61574 | GEO
Project description:Compared to what is known in model species, reproductive biology in citrus is still poorly understood. Although in recent years several efforts have been made to study pollen-pistil interaction and self-incompatibility, little information is available about the molecular mechanisms regulating these processes. We performed microarray analysis for the identification of candidate genes involved in pollen-pistil interaction and self-incompatibility in clementine (Citrus clementina Hort. ex Tan.). The analysis was performed comparing the transcriptome of laser-microdissected stylar canal cells isolated from two clementine genotypes differing for self-incompatibility response (‘Comune’, self-incompatible; and ‘Monreal’, a self compatible mutation of ‘Comune’).
2012-02-15 | GSE33014 | GEO
Project description:Jiashi melon and 86-1 melon were inoculated with Alternaria alternata, and the difference of gene expression was analyzed after 0, 6, 12, 18 and 24 days storage.
2024-03-01 | GSE226614 | GEO
Project description:Transcription regulator ACTR contributes pathogenicity through mediating ACT-toxin synthesis gene ACTS4 in Alternaria alternata
| PRJNA686686 | ENA
Project description:In mandarin (Citrus reticulata Blanco), rind separation is an essential trait for marketing, as it confers easy-peeling, an inheritable trait whose genetic basis has not yet been characterized. To this end, we used the 30 K Affymetrix Citrus GeneChip to compare gene expression in albedo tissues of an easy-peeling genotype (Clementine Nules) to a less easy-peeling hybrid genotype (Lee x Nova, USDA 88-2) at three time points: before, at and after the onset of rind separation. A high percent of genes were detected reliably by the chip (76.1 %), and Principal Component Analysis (PCA) based on these genes showed that three replicates were well clustered, indicating the reliability of the data set. Functional analysis of genes showing >5-fold difference in expression between Clementine Nules and Lee x Nova across three developmental points suggested that the transcriptome of the two varieties diverges as the maturation process advances. A pectin methylesterase was expressed at levels more than 100-fold higher in Clementine Nules than in Lee x Nova at all three time points and two genes encoding for pectinases were more than 10-fold higher in Clementine Nules than in Lee x Nova during the last sampling time. Different hydrolases, a glucanase and a carbohydrate kinase were higher in Nules than in Lee x Nova. Higher expression of two cellulose synthases, an expansin and an aquaporin was observed in the easy peel genotype Clementine Nules. The difference between Clementine Nules and Lee x Nova at the transcript level suggests that three main molecular mechanisms are involved in the easy peeling trait: 1) lower cell adhesion, 2) pronounced degradation of albedo cell wall polysaccharides, and 3) high and extended cell expansion rate of the rind. We used the 30 K Affymetrix Citrus GeneChip to compare gene expression in albedo tissues of an easy-peeling genotype (Clementine Nules) to a less easy-peeling hybrid genotype (Lee x Nova, USDA 88-2) at three time points (16 arrays): before, at and after the onset of rind separation.
2010-05-08 | E-GEOD-15886 | biostudies-arrayexpress
Project description:We have previously reported that the responses of tobacco tissues treated with AaGlucan are similar to those for laminarin [1]. To better understand the overall responses and signaling pathways in tobacco, we have identified here AaGlucan and laminarin early response genes, using a custom 16K BY-2 microarray [2,3]. BY-2 cells were treated with laminarin or an AaGlucan-enriched fraction prepared from A. alternata 102 culture-medium and subjected to microarray analysis. [1] Shinya, T., Menard, R., Kozone, I., Matsuoka, H., Shibuya, N., Kauffmann, S., Matsuoka, K. and Saito, M. (2006) Novel b-1,3-, 1,6-oligoglucan elicitor from Alternaria alternata 102 for defense responses in tobacco. FEBS J. 273: 2421-2431. [2] Matsuoka, K., Demura, T., Galis, I., Horiguchi, T., Sasaki, M., Tashiro, G. and Fukuda, H. (2004) A comprehensive gene expression analysis toward the understanding of growth and differentiation of tobacco BY-2 cells. Plant Cell Physiol. 45: 1280-1289. [3] Galis, I., Simek, P., Narisawa, T., Sasaki, M., Horiguchi, T., Fukuda, H. and Matsuoka, K. (2006) A novel R2R3 MYB transcription factor NtMYBJS1 is a methyl jasmonate-dependent regulator of phenylpropanoid-conjugate biosynthesis in tobacco. Plant J. 46: 573-592. AaGlucan was purified from Alternaria alternata 102 as a potent elicitor for tobacco [1] Four-day-old tobacco BY-2 cells after subculture were treated with 10 microg/ml AaGlucan enriched fraction for 1h. Total RNA was prepared from the control and treated samples and used for microarray experiment after reverse transcription and labeling with Cy5 fluorescent dye. [1] Shinya, T., Menard, R., Kozone, I., Matsuoka, H., Shibuya, N., Kauffmann, S., Matsuoka, K. and Saito, M. (2006) Novel b-1,3-, 1,6-oligoglucan elicitor from Alternaria alternata 102 for defense responses in tobacco. FEBS J. 273: 2421-2431.
2007-10-16 | E-GEOD-6948 | biostudies-arrayexpress