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Integrated Whole Transcriptome Profiling and Bioinformatics Analysis for researching lychee downy blight (RNA-Seq)

ABSTRACT: Integrated Whole Transcriptome Profiling and Bioinformatics Analysis for researching lychee downy blight (RNA-Seq)

PROVIDER: PRJNA922966 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR23050939_1.fastq.gz	Fastqsanger.gz
	SRR23050939_2.fastq.gz	Fastqsanger.gz
	SRR23050940_1.fastq.gz	Fastqsanger.gz
	SRR23050940_2.fastq.gz	Fastqsanger.gz
	SRR23050941_1.fastq.gz	Fastqsanger.gz

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Integrated Whole Transcriptome Profiling and Bioinformatics Analysis for researching lychee downy blight

Project description:Integrated Whole Transcriptome Profiling and Bioinformatics Analysis for researching lychee downy blight

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Integrated Whole Transcriptome Profiling and Bioinformatics Analysis for researching lychee downy blight (RNA-Seq)

Project description:Non coding RNA (ncRNA), such as miRNA, lncRNA and circRNA, has been found to play an important role in the mechanism of plant defense against pathogens. Lychee downy blingt is the main disease of litchi fruit, inflorescence and leaf, which seriously affects the yield of lychee. In this study, we carried out a P.litchii infection experiment on lychee fruits and leaves, and the mRNA and ncRNA in the treated fruits and leaves were analyzed by whole-transcriptome RNA sequencing technology. our work will Provide new insights for the resistance of lychee downy blight .

2023-02-06 | GSE222651 | GEO

Integrated Whole Transcriptome Profiling and Bioinformatics Analysis for researching lychee downy blight (miRNA-Seq)

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Transcription analysis of GW and SFZ lychees under lychee downy blight stress

Project description:Lychee (Litchi chinensis Sonn.) is a popular fruit worldwide. Lychee downy blight, caused by Peronophythora litchii (P. litchii), is one of the major diseases in lychee. In this study, we conducted a P. litchii infection experiment on the lychee leaves of GW and SFZ, and analyzed the mRNA in the treated leaves using transcriptome RNA sequencing technology. Our research will provide a preliminary basis for the prevention and control of lychee downy blight.

2024-09-22 | GSE262200 | GEO

Transcription analysis of GW and SFZ lychees under lychee downy blight stress

Project description:Transcription analysis of GW and SFZ lychees under lychee downy blight stress

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Integrated Whole Transcriptome Profiling and Bioinformatics Analysis for researching lychee downy blight

Project description:This SuperSeries is composed of the SubSeries listed below.

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Identification of signature genes of Dilated cardiomyopathy Using Integrated bioinformatics analysis

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Project description:Zeamines (ZMS), a class of polyamine-polyketide-nonribosomal peptide produced by bacterial isolate Dickeya zeae, were shown recently to be potent antibiotics against some bacterial pathogens. In this study, the results indicated that ZMS showed antifungal activity against Peronophythora litchii and other fungal pathogens. The activity of ZMS against the oomycete pathogen P. litchi, which causes the devastating litchi downy blight, was further investigated under in vitro and in vivo conditions. ZMS displayed potent inhibitory activity against the mycelial growth and sporangia germination of P. litchii. At a concentration of 2 μg/mL, about 99% of the sporangia germination was inhibited. Scanning electron microscopy and transmission electron microscopy analyses showed that treatment with ZMS could cause substantial damages to the oomycete endomembrane system. Furthermore, treatment of litchi fruits with ZMS solution significantly (P < 0.05) reduced the fruits decay and peel browning caused by P. litchii infection during storage at 28 °C. Taken together, our results provide useful clues on the antifungal mechanisms of ZMS, and highlight the promising potentials of ZMS as a fungicide, which in particular, may be useful for prevention and control of litchi fruits decay and browning caused by P. litchii infection during storage and transportation.

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Proteome responses of Grapevine to Downy mildew

Project description:A comparative proteomic analysis of grapevine leaves from the resistant genotype V. davidii ‘LiuBa-8’ (LB) and susceptible genotype V. vinifera ‘Pinot Noir’ (PN) at 12 hpi was conducted to understand the complex relationship between grapevine and P. viticola and the molecular mechanisms between difference resistant vitis genotypes at the early stage of infection. A total of 444 and 349 differentially expressed proteins (DEPs) were identified in LB and PN respectively at 12 hpi by iTRAQ. MapMan analysis showed that the majority of these DEPs were related to photosynthesis, metabolism, stress and redox. More up-expressed DEPs involved in photosynthesis and less down-expressed DEPs involved in metabolism contribute to the resistance in LB. PR10.2, PR10.3, HSF70.2 and HSP90.6 are proposed to be key proteins in both compatible and incompatible interactions. Accumulation H2O2 established the ROS signaling in incompatible interaction and APXs, GSTs maybe associated with the resistance of grapevine against downy mildew. Moreover, we verified four proteins to ensure the accuracy of proteome data using PRM. Overall, these data provide new insights into molecular events and provide valuable candidate proteins that could be used to illuminate molecular mechanisms underlying the incompatible and compatible interaction in early stage and eventually to be exploited to develop new protection strategy against downy mildew in grapevine.

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