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Gene expression profiles of pre-basophils versus mature basophils stimulated with antigen/IgE- or IL-3-stimulation.

ABSTRACT: Gene expression profiles of pre-basophils versus mature basophils stimulated with antigen/IgE- or IL-3-stimulation.

PROVIDER: PRJNA923235 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR23060635.fastq.gz	Fastqsanger.gz
	SRR23060636.fastq.gz	Fastqsanger.gz
	SRR23060637.fastq.gz	Fastqsanger.gz
	SRR23060638.fastq.gz	Fastqsanger.gz
	SRR23060639.fastq.gz	Fastqsanger.gz

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Gene expression profiles of pre-basophils versus mature basophils stimulated with antigen/IgE- or IL-3-stimulation.

Project description:Basophils are the least common granulocytes which represent less than 1% of peripheral blood leukocytes. Recent development of analytical tools for basophils enables us to understand their critical roles in allergic reactions and protection from parasitic infections. Nevertheless, the differentiation trajectory of basophils remains unclear. We have identified previously-unappreciated pre-basophil subpopulation which encompasses previously-defined basophil precursors (BaPs) in a series of experiments including scRNA-seq analysis (deposited to GSE206589). In this study, we explored gene expression profiles of pre-basophils and mature basophils activated by antigen/IgE-stimulation or IL-3-stimulation. We have revealed that IL-3 activated pre-basophils displayed distinct gene expression profiles from antigen/IgE- or IL-3-stimulated mature basophils, suggesting the unuque property of pre-basophils.

2023-03-21 | GSE222701 | GEO

SLAM-seq analysis of WT and TTP-KO basophils treated with antigen/IgE stimulation

Project description:Basophils play crucial roles in type 2 immune responses, such as chronic allergic inflammation and protective immunity against parasites. However, the molecular mechanisms underlying the regulation of proinflammatory molecule production by basophils remain unclear. To investigate the role of RNA-binding proteins (RBPs), we first analyzed the gene expression of CCCH zinc finger proteins in antigen/IgE-stimulated basophils and identified that Zfp36, encoding tristetraprolin (TTP), was the most significantly upregulated gene encoding a CCCH zinc finger protein. To explore the functions of TTP in basophils, we generated TTP-knockout (TTP-KO) mice using the CRISPR-Cas9 method and conducted bulk RNA-seq analysis of antigen/IgE-stimulated basophils from wild-type (WT) and TTP-KO mice. TTP-KO basophils exhibited elevated mRNA expression of pro-inflammatory mediators, such as Il4, Il13, Areg, Ccl3, and Cxcl2, compared to WT basophils, suggesting the key role of TTP in the expression of mRNAs encoding proinflammatory mediators. Since TTP is known to regulate mRNA stability in other immune cell types, we evaluated the mRNA stability of WT and TTP-KO basophils under antigen/IgE stimulation using SLAM-seq [thiol (SH)-Linked Alkylation for the Metabolic sequencing of RNA] analysis. SLAM-seq analysis revealed that the mRNA half-lives of proinflammatory molecules, such as Il4, Il13, Areg, Ccl3, and Cxcl2, were prolonged by TTP deficiency, suggesting the importance of TTP in regulating the stability of mRNAs encoding inflammatory molecules in basophils.

2024-11-17 | GSE272531 | GEO

Bulk RNA-seq analysis of WT and tristetraprolin (TTP)-knockout basophils treated with antigen/IgE stimulation

2024-11-17 | GSE272532 | GEO

Gene expression profiles of basophil progenitors, CD34- pre-basophils and mature basophils

Project description:In this study, we explored gene expression profiles of CD34-CLEC12Ahi pre-basophils isolated from the bone marrow as well as CD34-CLEC12Alo mature basophils from the bone marrow and CD34+ basophil progenitors. We revealed that the gene expression of mature basophils isolated from the bone marrow and spleen resembled each other whereas pre-basophils and mature basophils showed distinct gene expression profiles.

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Gene expression profiles of immature versus mature bone marrow-derived basophils (BMBAs) elicited by either IL-3 or TSLP.

Project description:In this study, we explored gene expression profiles of mature and immature basophils from either IL-3-elicited or TSLP-elicited BMBAs. We revealed that TSLP-elicited mature basophils displayed gene expression profile similar to that of IL-3-elicited mature rather than immature basophils.

2023-03-21 | GSE206592 | GEO

SLAM-seq analysis of WT and TTP-KO basophils treated with antigen/IgE stimulation

Project description:SLAM-seq analysis of WT and TTP-KO basophils treated with antigen/IgE stimulation

| PRJNA1137306 | ENA

Gene expression profiles of basophil progenitors, CD34- pre-basophils and mature basophils

Project description:Gene expression profiles of basophil progenitors, CD34- pre-basophils and mature basophils

| PRJNA851433 | ENA

Bulk RNA-seq analysis of WT and tristetraprolin (TTP)-knockout basophils treated with antigen/IgE stimulation

Project description:Bulk RNA-seq analysis of WT and tristetraprolin (TTP)-knockout basophils treated with antigen/IgE stimulation

| PRJNA1137305 | ENA

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Project description:The high-affinity Fc receptor for IgE, mainly present on mast cells and basophils, plays a crucial role in the development of allergic diseases. Monomeric IgE binding to receptor regulates mast cell survival, differentiation, and maturation. However, the underlying molecular mechanism remains unclear. Here we demonstrate that, prior to IgE binding, IgE receptor mostly exists as a homo-dimer on human mast cell membrane. The structure of human IgE receptor confirms the dimeric organization. Cholesterol-like molecules embedded within the transmembrane domain may stabilize the dimeric assembly. Upon IgE binding, the dimeric IgE receptor dissociates into two protomers, each binding to an IgE molecule. Importantly, this process elicits transcriptional activation of Egr1/3 and Ccl2 in rat basophils, which can be attenuated by inhibiting the IgE receptor dimer-to-monomer transition. Collectively, our study unveils the mechanism of antigen-independent, IgE-mediated receptor activation.

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Transcriptional heterogeneity of mast cells and basophils upon activation. [basophils]

Project description:We performed large-scale comparative microarrays of bone marrow -derived mast cells and basophils at rest, upon an adaptive-type action (IgE-crosslinking) or upon innate-type activation (IL-33-activation).

2017-03-17 | GSE96694 | GEO

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