Macaca mulatta
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA92349 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Circadian regulation of gene expression in central and peripheral tissue has been studied in mice. The biomedical implications of this findings led us to the development of a model in which to study the circadian mechanisms underlying primate physiology. The objective of this experiment has been to identify genes that are circadianly regulated in the monkey (macaca mulatta) adrenal gland. We analyzed the data using three filters: the first one for presence in at least three out of six time points (presence call=P, P value<0.05). The second one for high amplitude oscillations, using the coefficient of variation with a cutt of value of 0.2 (selecting probesets above that value). The last one for circadian expression, calculating the correlation of each curve to cosine curves of 24h periodicity and defined phases with increments of 10 minutes from 0 to 24h. After two filtrations, the probesets obtained were identified using gene ID, PubMed accession number, and description, and clustered into well-known pathways. Identification of clock genes was used as an internal control for the determination of phase distribution of the transcripts across the day. Part of the array data has been validated by sq PCR, real time-PCR and immunohistochemistry, with succesful results. This study provides evidences for circadian mechanisms of gene expression in primate tissue, in vivo.
2007-10-05 | E-GEOD-2703 | biostudies-arrayexpress
Project description:Circadian regulation of gene expression in central and peripheral tissue has been studied in mice. The biomedical implications of this findings led us to the development of a model in which to study the circadian mechanisms underlying primate physiology. The objective of this experiment has been to identify genes that are circadianly regulated in the monkey (macaca mulatta) adrenal gland. We analyzed the data using three filters: the first one for presence in at least three out of six time points (presence call=P, P value<0.05). The second one for high amplitude oscillations, using the coefficient of variation with a cutt of value of 0.2 (selecting probesets above that value). The last one for circadian expression, calculating the correlation of each curve to cosine curves of 24h periodicity and defined phases with increments of 10 minutes from 0 to 24h. After two filtrations, the probesets obtained were identified using gene ID, PubMed accession number, and description, and clustered into well-known pathways. Identification of clock genes was used as an internal control for the determination of phase distribution of the transcripts across the day. Part of the array data has been validated by sq PCR, real time-PCR and immunohistochemistry, with succesful results. This study provides evidences for circadian mechanisms of gene expression in primate tissue, in vivo. Keywords = primate Keywords = circadian rhythm Keywords = adrenal gland Keywords: time-course
2005-11-02 | GSE2703 | GEO
Project description:Gene Expression Profiling in Non-Human Primate Ileum after Total-Body Irradiation
| PRJNA299017 | ENA
Project description:Gene Expression Profiling in Non-Human Primate Jejunum after Total-Body Irradiation
| PRJNA299019 | ENA
Project description:Gene Expression Profiling in Non-Human Primate Colon after Total-Body Irradiation
| PRJNA299018 | ENA
Project description:Next Generation Sequencing Study of Circadian Changes in Transcriptome of Rhesus Macaque Pineal Gland and Retina
| PRJNA312819 | ENA
Project description:Pedigreed Primate Embryonic Stem Cells Express Homogeneous Familial Gene Profiles
| PRJNA99409 | ENA
Project description:The effects of LH ablation/replacement versus steroid ablation/replacement on gene expression in primate corpora lutea
| PRJNA113671 | ENA
Project description:Gene expression of lung tissues in non-human primate (NHP, rhesus macaque) after exposure to thoracic irradiation
| PRJNA289999 | ENA
Project description:Transcriptional architecture of the primate neocortex
| PRJNA145523 | ENA