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Candida albicans strain:101

ABSTRACT: Candida albicans strain 101 genome assembly

PROVIDER: PRJNA923600 | ENA |

REPOSITORIES: ENA

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			Action	DRS
		Other
	SRR30225897_subreads.fastq.gz	Fastqsanger.gz

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Candida albicans strain:101

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Project description:Differential expression between monosoic derivative and parental strain of Candida albicans. The important human pathogen Candida albicans possesses an unusual form of gene regulation, in which the copy number of an entire specific chromosome or a large portion of a specific chromosome changes in response to a specific adverse environment, thus, assuring survival. In the absence of the adverse environment, the altered portion of the genome can be restored to its normal condition. One major question is how C. albicans copes with gene imbalance arising by transitory aneuploid states. Here, we compared transcriptomes from two copies of chromosome 5 (Ch5) in a normal diploid strain 3153A and from a single copy of Ch5 in representative derivative Sor55. Statistical analysis revealed that at least 40% of transcripts from the monosomic Ch5 are fully compensated to a disomic level, thus, indicating the existence of a genome-wide mechanism maintaining cell homeostasis. However, a minor portion of transcripts diminished twofold in accordance with what would be expected for Ch5 monosomy. Another minor portion of transcripts, unexpectedly, increased up to twofold and higher then the disomic level, demonstrating indirect control by monosomy. We suggest that C. albicans unusual regulation of gene expression by the loss and gain of entire chromosomes is coupled with widespread compensation of gene dosage at the transcriptional level.

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Project description:Candida albicans BWP17 Wild-type strain was grown at 39oC to perform RNA deep sequencing analysis at the study: The chromatin state of Candida albicans pericentromeric repeats bears features of both euchromatin and heterochromatin. The aim of the study is to analyse differential gene expression at 30 oC and 39 oC of centromere proximal genes.

2016-06-23 | E-MTAB-4622 | biostudies-arrayexpress

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Project description:To investigate the diversity of gene contents of Candida albicans strain by array-based comparative genomic hybridization (array CGH; aCGH). A fluconazole-resistant Candida albicans strain CaLY350 was selected to carry out the comparative genomics microarray. Two-condition experiment, CaLY350 vs.SN152. Biological replicates: 2 control, 2 transfected, independently grown and harvested. One replicate per array.

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