Longitudinal analysis of the group A Streptococcus transcriptome
Ontology highlight
ABSTRACT:
OTHER RELATED OMICS DATASETS IN: GSE2713
PROVIDER: PRJNA92369 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Identification of the genetic events that contribute to host-pathogen interactions is important for understanding the natural history of infectious diseases and developing therapeutics. Transcriptome studies conducted on pathogens have been central to this goal in recent years. However, most of these investigations have focused on specific end points or disease phases, rather than analysis of the entire time course of infection. To gain a more complete understanding of how bacterial gene expression changes over time in a primate host, the transcriptome of group A Streptococcus (GAS) was analyzed during an 86-day infection protocol in 20 cynomolgus macaques with experimental pharyngitis. The study used 260 custom Affymetrix (Santa Clara, CA) chips, and data were confirmed by TaqMan analysis. Colonization, acute, and asymptomatic phases of disease were identified. Successful colonization and severe inflammation were significantly correlated with an early onset of superantigen gene expression. The differential expression of two-component regulators covR and spy0680 (M1_spy0874) was significantly associated with GAS colony-forming units, inflammation, and phases of disease. Prophage virulence gene expression and prophage induction occurred predominantly during high pathogen cell densities and acute inflammation. We discovered that temporal changes in the GAS transcriptome were integrally linked to the phase of clinical disease and host-defense response. Knowledge of the gene expression patterns characterizing each phase of pathogen-host interaction provides avenues for targeted investigation of proven and putative virulence factors and genes of unknown function and will assist vaccine research. Keywords: other
2005-06-17 | GSE2713 | GEO
Project description:This study evaluate how catabolite control protein A (CcpA) inactivation affected gene transcript levels in the group A Streptococcus serotype M1 strain MGAS5005 during growth in standard laboratory medium. Keywords: transcriptome analysis
2008-01-30 | GSE10156 | GEO
Project description:Relatively little is understood about the dynamics of global hostâpathogen transcriptome changes that occur during bacterial infection of mucosal surfaces. To test the hypothesis that group A Streptococcus (GAS) infection of the oropharynx provokes a host transcriptome response, we performed genome-wide transcriptome analysis using a nonhuman primate model of experimental pharyngitis. We also identified host and pathogen biological processes and individual host and pathogen gene pairs with correlated patterns of expression, suggesting interaction. For this study, 509 host genes and seven biological pathways were differentially expressed throughout the entire 32-day infection cycle. GAS infection produced an initial widespread significant decrease in expression of many host genes, including those involved in cytokine production, vesicle formation, metabolism, and signal transduction. This repression lasted until day 4, at which time a large increase in expression of host genes was observed, including those involved in protein translation, antigen presentation, and GTP-mediated signaling. The interactome analysis identified 73 host and pathogen gene pairs with correlated expression levels. We discovered significant correlations between transcripts of GAS genes involved in hyaluronic capsule production and host endocytic vesicle formation, GAS GTPases and host fibrinolytic genes, and GAS response to interaction with neutrophils. We also identified a strong signal, suggesting interaction between host γδ T cells and genes in the GAS mevalonic acid synthesis pathway responsible for production of isopentenyl-pyrophosphate, a short-chain phospholipid that stimulates these T cells. Taken together, our Q:2 results are unique in providing a comprehensive understanding of the hostâpathogen interactome during mucosal infection by a bacterial pathogen. Longitudinal pharyngeal infection of cynomolgus macaques by group A Streptococcus Briefly, animals were GAS-culture negative and had negligible antistreptolysin O titers, indicating no recent history of GAS exposure. Twenty animals were subjected to a mock-inoculation protocol (PBS only) for 5 weeks, rested for 4 weeks, and inoculated in the upper respiratory tract with 107 CFUsMGAS5005. Blood, saliva, and throat swabs were collected on days 0, 1, 2, 4, 7, 9, 16, 23, 32, 45, 58, 72, and 86. Only the first nine time-points were studied because specimens collected during days 0 to 32 had matching comparator specimens from the mock-infection protocol. Thirty-two clinical and laboratory parameters were measured by the same veterinarian during mock and infection periods. Array data was only for the tonsil swabs, while blood and saliva used for other tests.
2010-03-18 | E-GEOD-20262 | biostudies-arrayexpress
Project description:This study evaluate how catabolite control protein A (CcpA) inactivation affected gene transcript levels in the group A Streptococcus serotype M1 strain MGAS5005 during growth in standard laboratory medium. Keywords: transcriptome analysis 4 replicates of strain MGAS5005 and delta_cppA were grown in THY to early and late logarithmic growth phases. The transcriptomes of each samples were then analyzed.
2010-05-26 | E-GEOD-10156 | biostudies-arrayexpress
Project description:Relatively little is understood about the dynamics of global host–pathogen transcriptome changes that occur during bacterial infection of mucosal surfaces. To test the hypothesis that group A Streptococcus (GAS) infection of the oropharynx provokes a host transcriptome response, we performed genome-wide transcriptome analysis using a nonhuman primate model of experimental pharyngitis. We also identified host and pathogen biological processes and individual host and pathogen gene pairs with correlated patterns of expression, suggesting interaction. For this study, 509 host genes and seven biological pathways were differentially expressed throughout the entire 32-day infection cycle. GAS infection produced an initial widespread significant decrease in expression of many host genes, including those involved in cytokine production, vesicle formation, metabolism, and signal transduction. This repression lasted until day 4, at which time a large increase in expression of host genes was observed, including those involved in protein translation, antigen presentation, and GTP-mediated signaling. The interactome analysis identified 73 host and pathogen gene pairs with correlated expression levels. We discovered significant correlations between transcripts of GAS genes involved in hyaluronic capsule production and host endocytic vesicle formation, GAS GTPases and host fibrinolytic genes, and GAS response to interaction with neutrophils. We also identified a strong signal, suggesting interaction between host γδ T cells and genes in the GAS mevalonic acid synthesis pathway responsible for production of isopentenyl-pyrophosphate, a short-chain phospholipid that stimulates these T cells. Taken together, our Q:2 results are unique in providing a comprehensive understanding of the host–pathogen interactome during mucosal infection by a bacterial pathogen.
2010-03-18 | GSE20262 | GEO
Project description:Transcriptome analysis of of wt and PerR mutant Group A streptococcus strain 003Sm grown in the presence or absence of hydrogen peroxide, at early and late log
2011-12-31 | E-MTAB-628 | biostudies-arrayexpress
Project description:Transcriptome analysis of of wt and PerR mutant Group A streptococcus strain 003Sm grown in the presence or absence of hydrogen peroxide, at early and late log
2011-11-18 | E-MTAB-646 | biostudies-arrayexpress
Project description:Group B Streptococcus (GBS), in its transition from commensal to pathogen, will encounter diverse host environments and thus require coordinately controlling its transcriptional responses to these changes. This work was aimed at better understanding the role of two component signal transduction systems (TCS) in GBS pathophysiology through a systematic screening procedure. We first performed a complete inventory and sensory mechanism classification of all putative GBS TCS by genomic analysis. Five TCS were further investigated by the generation of knock-out strains from WT strain CJB111, and in vitro transcriptome analysis identified genes regulated by these systems.
2014-06-03 | E-MTAB-2423 | biostudies-arrayexpress
Project description:This SuperSeries is composed of the following subset Series: GSE17767: Transcriptome comparison of Streptococcus pyogenes strain MGAS2221 with that of an isogenic PEL mutant GSE17789: Tiling microarray-based identification of small regulatory RNAs in the group A Streptococcus (GAS) Refer to individual Series
2009-08-25 | E-GEOD-17790 | biostudies-arrayexpress
Project description:Immune responses to group A streptococcus in humans can lead to the development of acute rheumatic fever and rheumatic heart disease. Immune pathways that are activated by group A streptococcus are potential targets for inhibiting autoimmune responses to group A streptococcus. This experiment tests the impact of the drug hydroxychloroquine on immune responses to group A streptococcus in peripheral blood mononuclear cells
2016-10-21 | GSE74235 | GEO