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Effect of TET3 expression in macrophage

ABSTRACT: Effect of TET3 expression in macrophage

PROVIDER: PRJNA924837 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR23103413_1.fastq.gz	Fastqsanger.gz
	SRR23103413_2.fastq.gz	Fastqsanger.gz
	SRR23103414_1.fastq.gz	Fastqsanger.gz
	SRR23103414_2.fastq.gz	Fastqsanger.gz
	SRR23103415_1.fastq.gz	Fastqsanger.gz

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Effect of TET3 expression in macrophage

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Effect of TET3 expression on muscle insulin sensitivity

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Rest recruits neuronal Tet3 to induce chromatin remodeling and gene expression

Project description:Ten-eleven translocation (Tet) hydroxylases (Tet1-3) oxidize 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC). In neurons increased 5hmC levels within gene bodies correlate positively with gene expression. The mechanisms controlling Tet activity and 5hmC levels are poorly understood. In particular, it is not known how the neuronal Tet3 isoform lacking a DNA binding domain is targeted to the DNA. To identify factors binding to Tet3 we screened for proteins that co-precipitate with Tet3 from mouse retina and identified the transcriptional repressor Rest as a highly enriched Tet3-specific interactor. Rest was able to enhance Tet3 hydroxylase activity after co-expression and overexpression of Tet3 activated transcription of Rest-target genes. Moreover, we found that Tet3 also interacts with Nsd3 and two other H3K36 methyltransferases and is able to induce H3K36 trimethylation. We propose a mechanism for transcriptional activation in neurons that involves Rest-guided targeting of Tet3 to the DNA for directed 5hmC-generation and Nsd3-mediated H3K36 trimethylation.

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Project description:PGC7 is a primordial germ cell (PGCs)-specific protein involved in epigenetic chromatin reprogramming in the zygote following fertilization. TET3 is a dioxygenase that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC) and plays a key role in epigenetic chromatin reprogramming in the zygote following fertilization. PGC7 participates in protection of DNA methylation in the maternal pronucleus by preventing conversion of 5mC to 5hmC by TET3 oxidization subsequent DNA demethylation. Thus we tested the co-localization of PGC7 and TET3 in specific loci by ChIP-seq assays.

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Effect of depletion of TET3 on gene expression of HTR8-SVneo trophoblast cells.

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Redirected nuclear glutamate dehydrogenase supplies Tet3 with a-ketoglutarate in neurons

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