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Different biological roles of embryonic- and monocyte-derived macrophages in ovarian cancer ascites ecosystem.

ABSTRACT: Different biological roles of embryonic- and monocyte-derived macrophages in ovarian cancer ascites ecosystem.

PROVIDER: PRJNA925023 | ENA |

REPOSITORIES: ENA

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Different biological roles of embryonic- and monocyte-derived macrophages in ovarian cancer ascites ecosystem.

Project description:To investigate the dichotomous ontogeny of ascites-enriched macrophages and the contribution of embryonic-derived macrophages to the ascites microenvironment and tumor progression.

2023-05-05 | GSE223121 | GEO

Mixed-polarization phenotype of ascites-associated macrophagesin human ovarian carcinoma: correlation of CD163 expression, cytokine levels and early relapse

Project description:Ascites-associated macrophages were collected from human ovarian carcinoma patients and their expression profiles determined via Agilent microarrays. In addition, monocyte derived macrophages from healthy donors were differentiated ex-vivo with MCSF and GMCSF and their expression profiles determined as well.

2014-03-07 | E-MTAB-1661 | biostudies-arrayexpress

Ascites enriches for ovarian cancer stem-like cells that express membrane GRP78

Project description:Ovarian cancer patients are generally diagnosed at stage III/IV, when ascites is common. The volume of ascites positively correlates with the extent of metastasis and negatively with prognosis. Membrane GRP78, a stress-inducible endoplasmic reticulum chaperone which also appears on the plasma membrane (memGRP78) of aggressive cancers, plays a crucial role in the maintenance of embryonic stem cells. Our present study demonstrates that tumor cells isolated from ascites generated by epithelial ovarian cancer (ID8 cells) bearing mice have increased memGRP78 expression compared to ID8 cells in normal culture. We hypothesize that these ascites associated memGRP78+ cells are cancer stem-like cells (CSC) and memGRP78 is functionally important in CSCs. Supporting this hypothesis, we show that memGRP78+ cells isolated from ascites have increased sphere forming and tumor initiating abilities compared to memGRP78- cells. When the tumor microenvironment is recapitulated by adding ascites fluid to cell culture, ID8 cells express more memGRP78 and increased self-renewing ability compared to those cultured in medium alone. Moreover, compared to their counterparts cultured in normal medium, ID8 cells cultured in ascites, or isolated from ascites, show an increased expression of stem cell markers Sca-1, Snail and SOX9. Importantly, antibodies directed against the carboxy (COOH)-terminal domain of GRP78 significantly reduce the self-renewing ability of murine and human ovarian cancer cells pre-incubated with ascites, associated with a decreased phosphorylation of Akt and GSK3α, and reduced level of the transcriptional factor Snail. Based on this data, we suggest that memGRP78 is a logical therapeutic target for late stage ovarian cancer.

2015-03-09 | GSE61285 | GEO

RNAseq of ovarian cancer ascites cells

Project description:RNAseq of ovarian cancer ascites cells

| PRJEB19616 | ENA

Ascites enriches for ovarian cancer stem-like cells that express membrane GRP78

2015-03-09 | E-GEOD-61285 | biostudies-arrayexpress

single cell RNA-seq analysis of ovarian cancer ascites

Project description:To understand the cellular diversity within malignant ascites, we profiled ~11,000 single cells from 22 samples by Smartseq2 and by 10x.

2020-05-01 | GSE146026 | GEO

Ascites exosomal lncRNA PLADE enhances platinum sensitivity by inducing R-loop in ovarian cancer

Project description:To investigate functional lncRNAs in ovarian cancer ascites exosomes, we performed high-throughput RNA sequencing (RNA-seq) of ascites-derived exosomes from 3 cisplatin-sensitive and 7 cisplatin-resistant HGSOC patients

2023-11-15 | GSE247337 | GEO

Genome-wide analysis of mRNA expression alterations in Stage III-IV high-grade serous ovarian cancer patients exhibiting clinically defined differences in ascites volume (high volume ascites versus low volume ascites)

Project description:Microarrays were used to examine gene expression changes in the surgical resections of high-grade serous ovarian cancer patients exhibiting clinically distinct levels of ascites volume. The present studies primary aim was to determine if there is a molecular gene expression difference between the patients presenting at time of surgery when high volumes ascites cases were compared to those with low volume ascites. The secondary aim was to determine what relevance this difference, if found, has to previously discovered molecular sub-types of high grade serous ovarian cancer. Total RNA obtained from snap-frozen stage III-IV high-grade serous ovarian cancer patients presenting with low volume (<=200 cc) or high volume (>=1000 cc) ascites volume.

2014-10-01 | E-GEOD-51831 | biostudies-arrayexpress

Ascites-derived circulating microRNAs as potential diagnostic biomarkers of gastric cancer-associated malignant ascites

Project description:Peritoneal carcinomatosis with malignant ascites is associated with dismal prognosis in gastric cancer. Malignant ascites is the most relevant body fluid in which to seek diagnostic biomarkers for peritoneal carcinomatosis. We aimed to identify and validate ascites-derived circulating microRNAs (miRNAs) that are differentially expressed between liver cirrhosis-associated benign ascites (LC-ascites) and gastric cancer-associated malignant ascites (GC-ascites). MiRNA expression levels were investigated in three independent cohorts. Overall, 165 ascites samples (73 LC-ascites and 92 GC-ascites) were obtained from the National Biobank of Korea. Initially, microarrays were used to screen the expression levels of 2,006 miRNAs in the discovery cohort (n = 22). Subsequently, quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) analyses were used to validate the expression levels of selected miRNAs in the training (n = 70) and validation (n= 73) cohorts. In addition, the levels of carcinoembryonic antigen (CEA), a commonly used tumor marker, were determined in the ascites samples. Expression levels of miR-574-3p, miR-181b-5p, miR-4481, and miR-181d were significantly lower in the GC-ascites samples than in the LC-ascites samples, and miR-181b-5p showed the best diagnostic performance for GC-ascites (area under the curve [AUC] = 0.798 and 0.846 for the training and validation cohorts, respectively). The diagnostic performance of CEA for GC-ascites was improved if CEA and miR-181b-5p were analyzed together (AUC = 0.981 and 0.946 for the training and validation cohorts, respectively). Overall, we identified ascites-derived circulating miRNAs capable of differentiating non-malignant ascites and GC-ascites, and demonstrated that the combined use of miR-181b-5p and CEA produces the optimal diagnostic yield.

2019-02-12 | GSE126399 | GEO

2014-10-01 | GSE51831 | GEO

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