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The transcriptomic profile of primary cells isolated from intestinal muscle layers in 2D or 3D culture conditions and with different culture media

ABSTRACT: The transcriptomic profile of primary cells isolated from intestinal muscle layers in 2D or 3D culture conditions and with different culture media

PROVIDER: PRJNA925141 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR23110206.fastq.gz	Fastqsanger.gz
	SRR23110207.fastq.gz	Fastqsanger.gz
	SRR23110208.fastq.gz	Fastqsanger.gz
	SRR23110209.fastq.gz	Fastqsanger.gz
	SRR23110210.fastq.gz	Fastqsanger.gz

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The transcriptomic profile of primary cells isolated from intestinal muscle layers in 2D or 3D culture conditions and with different culture media

Project description:The intestinal muscle layers execute various gut wall movements to achieve controlled propulsion and mixing of intestinal content. Engineering intestinal muscle layers with complex contractile function is critical for developing bioartificial intestinal tissue to treat patients with short bowel syndrome. A living intestinal muscle patch is created for the first time in vitro to generate three distinct motility patterns and display multiple digesta manipulations. Developing such intestinal muscle patches combines a differentiation medium and a 3D scaffold. Next-generation RNA sequencing was performed across primary cells isolated from the intestinal muscle layers that were further cultured with/without the differentiation medium or with/without the 3D scaffold to characterize the effect of the medium and the scaffold on the transcriptomic profile of the cells. We found that the successful generation of the contracting intestinal muscle patch relies on both biological factors in the medium and environmental cues from the scaffold. In cooperation, the medium and the scaffold both greatly enhance the differentiation and maturity of the enteric neurons. Separately, the medium profoundly impacts ion transport activities. The scaffold markedly reshapes cell communication and protein binding.

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