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LuxR402 of Novosphingobium sp. HR1a regulates the correct configuration of cell envelopes

ABSTRACT: LuxR402 of Novosphingobium sp. HR1a regulates the correct configuration of cell envelopes

PROVIDER: PRJNA925480 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR23128787_1.fastq.gz	Fastqsanger.gz
	SRR23128787_2.fastq.gz	Fastqsanger.gz
	SRR23128788_1.fastq.gz	Fastqsanger.gz
	SRR23128788_2.fastq.gz	Fastqsanger.gz
	SRR23128789_1.fastq.gz	Fastqsanger.gz

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Similar Datasets

LuxR402 of Novosphingobium sp. HR1a regulates the correct configuration of cell envelopes

Project description:In this work, we report that the knockout mutant in other of this luxR-like genes (named luxR402) in Novosphingobium sp. HR1a; floculated faster than the wild-type when cultures are in repose. Transcriptomic analysis allowed us to determine the LuxR402 regulon; we have identified that the carbohydrate assimilation pathway and TCA cycle are affected in the mutant. Accordingly, the mutant presented poorer growth than the wild-type when growing in different carbon sources. At stationary phase of growth the pili biosynthesis, trehalose utilization and capside-related proteins were affected in the mutant strain. Microscopy assays determined that the mutant strain cultures presented cells aggrupations and that the extracellular matrix is less abundant than in the wild-type cultures.

2023-07-13 | GSE223267 | GEO

Metabolic capacities of Novosphingobium sp. HR1a in the rhizosphere

Project description:Rhizoremediation, the biotechnology of the utilization of rhizospheric microorganisms associated with plant roots for the elimination of soil contaminants, is based on the ability of microorganisms to metabolize nutrients from plant root exudates, in order to survive the stressful conditions of the rhizosphere, and thereby, to co-metabolize or even mineralize toxic environmental contaminants. Novosphingobium sp. HR1a is a bacterial strain able to degrade a wide variety of polycyclic aromatic hydrocarbons (PAHs). We have demonstrated that this bacterium is able to grow in vegetated microcosms and to eliminate phenanthrene in the presence of clover faster than in non-vegetated systems, establishing a positive interaction with clover. We have studied the molecular basis of this interaction by phenomic, metabolomic and transcriptomic analyses, demonstrating that the positive interaction between clover and Novosphingobium sp. HR1a is a result of the bacterial utilization of different carbon and nitrogen sources (such as sugars, amino acids and organic acids) released during seedling development, and the capacity of exudates to induce the PAH degradation pathway. These results are pointing out to Novosphingobium sp. HR1a as a promising strain for the bioremediation of PAH-contaminated soils.

2021-04-19 | GSE172273 | GEO

PahT regulates carbon fluxes in Novosphingobium sp. HR1a and influence its survival in soil and rhizosphere.

Project description:In this work we describe the role of paht gene from Novosphingobium sp. HR1a, as a regulator that seems to be involved in the control over the utilization of carbon and sulfur sources mainly involved in the control of pyruvate production and in the acetyl-CoA biosynthesis and PEP recycling from pyruvate.

2021-07-01 | GSE163593 | GEO

LuxR402 of <i>Novosphingobium</i> sp. HR1a regulates the correct configuration of cell envelopes.

Project description:Although there is some evidence to suggest that LuxR-solo proteins participate in inter-species or even inter-kingdom communication, most of the LuxR-solo protein functions are unknown. We have characterized the LuxR402 regulator of Novosphingobium sp. HR1a, a bacterial strain with the ability to establish high numbers in the plant rhizosphere and able to degrade a wide range of polycyclic aromatic hydrocarbons. LuxR402 controls the aggregation state of the bacterial culture; cultures of a mutant strain lacking this regulator flocculate in less than 3 h without agitation. We have demonstrated that the bacterial surface of the mutant is highly hydrophobic and that the mutant cells assimilate sugars slower than the wild-type. The flocculation mechanism has been demonstrated to be involved in the survival of the strain under unfavorable conditions; the luxR402 gene is repressed and produces flocculation in the presence of salicylate, a substrate that, although being assimilated by Novosphingobium, is toxic to cells at high concentrations. The flocculation of cultures in industrial setups has mainly been achieved through the addition of chemicals; these studies open up the possibility of controlling the flocculation by regulating the level of expression of the luxR402 gene.

| S-EPMC10413115 | biostudies-literature

Novosphingobium sp. HR1a

Project description:Novosphingobium sp. HR1a Genome sequencing and assembly

| PRJNA641101 | ENA

Metabolic capacities of Novosphingobium sp. HR1a in the rhizosphere

Project description:Metabolic capacities of Novosphingobium sp. HR1a in the rhizosphere

| PRJNA722733 | ENA

PahT regulates carbon fluxes in Novosphingobium sp. HR1a and influence its survival in soil and rhizosphere.

Project description:PahT regulates carbon fluxes in Novosphingobium sp. HR1a and influence its survival in soil and rhizosphere.

| PRJNA686887 | ENA

PahT regulates carbon fluxes in Novosphingobium sp. HR1a and influences its survival in soil and rhizospheres.

Project description:Novosphingobium sp. HR1a is a good biodegrader of PAHs and aromatic compounds, and also a good colonizer of rhizospheric environments. It was previously demonstrated that this microbe is able to co-metabolize nutrients existing in root exudates together with the PAHs. We have revealed here that PahT, a regulator of the IclR-family, regulates the central carbon fluxes favouring the degradation of PAHs and mono-aromatic compounds, the ethanol and acetate metabolism and the uptake, phosphorylation and further degradation of mono- and oligo-saccharides through a phosphoenolpyruvate transferase system (PTS). As final products of these fluxes, pyruvate and acetyl-CoA are obtained. The pahT gene is located within a genomic region containing two putative transposons that carry all the genes for PAH catabolism; PahT also regulates these genes. Furthermore, encoded in this genomic region, there are genes that are involved in the recycling of phosphoenolpyruvate, from the obtained pyruvate, which is the motor molecule involved in the saccharide uptake by the PTS system. The co-metabolism of PAHs with different carbon sources, together with the activation of the thiosulfate utilization and an alternative cytochrome oxidase system, also regulated by PahT, represents an advantage for Novosphingobium sp. HR1a to survive in rhizospheric environments.

| S-EPMC8360164 | biostudies-literature

Aromatic dimer dehydrogenases from Novosphingobium aromaticivorans reduce aromatic diketones

Project description:Aromatic diketones are a major product of formic acid lignin depolymerization. Novosphingobium aromaticivorans can degrade these diketones, but the enzymes used in this process were unknown. We used RNA-Seq to identify aromatic dimer dehydrogenases as potential candidates for the initial reduction of the aromatic G-diketone, then verified this using in vitro enzyme assays.

2021-05-22 | GSE174697 | GEO

Novosphingobium sp. HR1a modifies the clover-root architecture through the alteration of the jasmonic acid levels in clover plants

Project description:Novosphingobium sp. HR1a modifies the clover-root architecture through the alteration of the jasmonic acid levels in clover plants

| PRJNA902342 | ENA

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