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S-adenosylmethionine synthases specify distinct H3K3me3 populations and gene expression patterns during heat stress [RNA-seq: sams-4 RNAi, Heat shock]

ABSTRACT: S-adenosylmethionine synthases specify distinct H3K3me3 populations and gene expression patterns during heat stress [RNA-seq: sams-4 RNAi, Heat shock]

PROVIDER: PRJNA926941 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR23199402.fastq.gz	Fastqsanger.gz
	SRR23199403.fastq.gz	Fastqsanger.gz
	SRR23199404.fastq.gz	Fastqsanger.gz
	SRR23199405.fastq.gz	Fastqsanger.gz
	SRR23199406.fastq.gz	Fastqsanger.gz

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S-adenosylmethionine synthases specify distinct H3K3me3 populations and gene expression patterns during heat stress

Project description:S-adenosylmethionine synthases specify distinct H3K3me3 populations and gene expression patterns during heat stress

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S-adenosylmethionine synthases specify distinct H3K3me3 populations and gene expression patterns during heat stress [RNA-seq: sams RNAi, 20C]

Project description:S-adenosylmethionine synthases specify distinct H3K3me3 populations and gene expression patterns during heat stress [RNA-seq: sams RNAi, 20C]

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S-adenosylmethionine synthases specify distinct H3K3me3 populations and gene expression patterns during heat stress [CUT&Tag]

Project description:We find that two SAM synthases in C. elegans, SAMS-1 and SAMS-3/4, contribute differently to modification of H3K4me3, gene expression and survival in the heat stress response. Our data showing differential SAM provisioning to distinct groups of methylated targets based on SAM synthase source suggests that determining how methyl donors are provided will broaden insight into 1CC functions in aging and stress.

2023-02-13 | GSE223594 | GEO

S-adenosylmethionine synthases specify distinct H3K3me3 populations and gene expression patterns during heat stress [CUT&Tag]

Project description:S-adenosylmethionine synthases specify distinct H3K3me3 populations and gene expression patterns during heat stress [CUT&Tag]

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S-adenosylmethionine synthases specify distinct H3K3me3 populations and gene expression patterns during heat stress [RNA-seq: sams-4 RNAi, Heat shock]

2023-02-13 | GSE223596 | GEO

S-adenosylmethionine synthases specify distinct H3K3me3 populations and gene expression patterns during heat stress [RNA-seq: sams RNAi, 20C]

2023-02-13 | GSE223595 | GEO

S-adenosylmethionine synthases specify distinct H3K3me3 populations and gene expression patterns during heat stress

Project description:This SuperSeries is composed of the SubSeries listed below.

2023-02-13 | GSE223597 | GEO

Comparison of heat responsive gene expression after histone methyltransferase RNAi in C. elegans

Project description:We resport the changes in gene expression occuring after heat exposure in C. elegans with low s-adenosylmethionine, or RNAi of two H3K4 methyltransferases Using a C. elegans model of low SAM, we previously found that transcriptional responses response to a bacterial pathogen failed and these bacterial-response genes did not show normal H3K4me3 close to the transcriptional start sites, (Ding et al. 2015 Cell Metabolism). We also found the HMT set-16/MLL was required for full induction, whereas set-2/SET1 appeared dispensable (Ding et al. 2015 Cell Metabolism). We hypothesized that animals with low SAM might fail to transcriptionally respond to stress and that the HMTs may also have distinct roles in modulating stress responses. In our present study, we set out to compare induction of transcriptional responses and survival upon stress exposure between C. elegans with reduced SAM (sams-1(RNAi)) and animals with limited H3K4me3 function, set-2/SET1, and set-16/MLL RNAi. Because distinct stresses may rely on different transcriptional activation mechanisms, we also compared whole-genome expression patterns in three stresses: pathogenic bacteria (PA14), xenotoxic (R24) and heat in response to each RNAi.

2018-10-20 | GSE121510 | GEO

S-adenosylmethionine synthases specify distinct H3K4me3 populations and gene expression patterns during heat stress.

Project description:Methylation is a widely occurring modification that requires the methyl donor S-adenosylmethionine (SAM) and acts in regulation of gene expression and other processes. SAM is synthesized from methionine, which is imported or generated through the 1-carbon cycle (1 CC). Alterations in 1 CC function have clear effects on lifespan and stress responses, but the wide distribution of this modification has made identification of specific mechanistic links difficult. Exploiting a dynamic stress-induced transcription model, we find that two SAM synthases in Caenorhabditis elegans, SAMS-1 and SAMS-4, contribute differently to modification of H3K4me3, gene expression and survival. We find that sams-4 enhances H3K4me3 in heat shocked animals lacking sams-1, however, sams-1 cannot compensate for sams-4, which is required to survive heat stress. This suggests that the regulatory functions of SAM depend on its enzymatic source and that provisioning of SAM may be an important regulatory step linking 1 CC function to phenotypes in aging and stress.

| S-EPMC9984191 | biostudies-literature

The effect of Bcl-xL overexpression on H3K3me3

Project description:The effect of Bcl-xL overexpression on H3K3me3

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