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ScRNA-sequencing of mesenchymal cells and immune cells sorted from the lung of PBS or HDM challenged mice

ABSTRACT: scRNA-sequencing of mesenchymal cells and immune cells sorted from the lung of PBS or HDM challenged mice

PROVIDER: PRJNA928691 | ENA |

REPOSITORIES: ENA

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			Action	DRS
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	SRR23253200.fastq.gz	Fastqsanger.gz
	SRR23253201.fastq.gz	Fastqsanger.gz
	SRR23253202.fastq.gz	Fastqsanger.gz
	SRR23253203.fastq.gz	Fastqsanger.gz

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scRNA-sequencing of mesenchymal cells and immune cells sorted from the lung of PBS or HDM challenged mice

Project description:We compared the transcriptomes of mesenchymal cells and immune cells in mice sensitized and challenged with house dust mite (HDM) extracts and those in control PBS-treated mice, using single cell RNA-seq (scRNA-seq). Data with mesenchymal cells and data with immune cells were obtained from separate experiments. For scRNA-seq of mesenchymal cells, each sample was pooled from cells from 3 mice and 2 different samples per group (for a total of 6 mice) were used. For scRNA-seq of immune cells, each sample was from cells of one individual mouse.

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Transcriptomic analysis in airway epithelial cells in HDM-sensitized STAT3-WT and STAT3-cKO mice

Project description:Purpose: To elucidate the roles of airway epithelial STAT3 in allergic airway inflammation. Method: Doxycycline-induced airway epithelial cells-specific STAT3-deficient mice (STAT3-cKO) and their genetic control mice (STAT3-WT) were sensitized intratracheally twice with house dust mite (HDM) extract or PBS at a 7-day interval. Airway epithelial cells were isolated by a cell sorter 48 h after the last senstization. N=2 each. Results: Using an optimized data analysis workflow, 206 transcripts showed differential expression between HDM-treated STAT3-WT and STAT3-cKO with adjusted p value <0.05.

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Project description:Our study investigates the activation and transcriptional programming of primary skin cells (FACS-purified Langerhans cells and single cell analysis of the whole biopsy) from patients with atopic dermatitis (n=22, nr = rensponding, nnr=nonresponding), exposed to a control or HDM patch test. 6 mm biopsies were taken from control and HDM patch test site 48h post application.

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