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Drosophila melanogaster

ABSTRACT: Identification of 20E-regulated genes in Drosophila cultured larval organs

OTHER RELATED OMICS DATASETS IN: E-GEOD-3069

PROVIDER: PRJNA93147 | ENA |

REPOSITORIES: ENA

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Transcription profiling of Drosophila melanogaster third instar larvae organ cultures treated with 20-hydroxyecdysone, cycloheximide vs controlds to identify 20-hydroxyecdysone regulated genes at the onset of metamorphosis

Project description:To identify 20E-regulated genes, wandering third instar larvae were dissected and their organs were cultured in the presence of either no hormone, 20E alone, cycloheximide alone, or 20E plus cycloheximide for six hours. Experiment Overall Design: Eight partial blue gut third instar larvae were dissected in each well of a 9 well glass dish (Corning) and cultured in ~100 Âµl oxygenated Schneiders Drosophila Medium (Gibco) at 25Â°C. Cultures were incubated in an a styrofoam box under a constant flow of oxygen. Following an initial incubation of 1 hour, the medium was removed and replaced with either fresh Schneiders Drosophila Medium (no hormone), medium plus 8.5x10-5 M cycloheximide (Sigma), medium plus 5x10-6 M 20-hydroxyecdysone (Sigma), or medium plus cycloheximide and 20E, each for 6 hrs at 25Â°C. Organs were collected and RNA extracted from these samples was analyzed on Affymetrix Drosophila Genome Arrays.

2007-10-31 | E-GEOD-3060 | biostudies-arrayexpress

Identification of 20E-regulated genes in Drosophila cultured larval organs

2005-10-04 | GSE3060 | GEO

Drosophila melanogaster

Project description:Identification of Drosophila MBF1 regulated genes in embryos

| PRJNA300938 | ENA

Drosophila melanogaster

Project description:Identification of Drosophila Mid regulated genes in embryos

| PRJNA297945 | ENA

Transcriptomic analysis of Drosophila imaginal wing discs cultured in insulin or 20-hydroxyecdysone

Project description:We identified a role for low concentrations of 20-hydroxyecdysone (20E) in promoting growth of the Drosophila larval wing imaginal disc using genetics and in vitro explant culture. We found that 20E alone could promote proliferation in the absence of added insulin, a well known regulator of growth. To investigate the mechanism by which these hormones promote growth, we sequenced the transcriptomes of mid-third instar wing discs cultured in no hormone, 20E alone or insulin alone at two timepoints (4hr and 9hr). We also sequenced the transcriptomes of freshly dissected wing discs (not cultured). We found that within 4hr of culture without hormone, the expression of 1604 genes goes down and 1409 genes goes up, relative to uncultured discs. Changes in the expression of 1911 genes could be prevented, at least transiently, by the addition of either 20E or insulin. The transcriptional response to insulin was remarkably transient, decreasing by rougly 50% from 4hr to 9hr of culture. The response to 20E was smaller than that induced by insulin, but longer lasting. Insulin-induced genes are most significantly enriched in GO-terms describing DNA replication, ribosome biogenesis, translation, purine biosynthesis, and mitochondrial biogenesis. Insulin-repressed genes are enriched in genes involved in autophagy and cell death. 20E-regulated genes were also enriched for genes involved in DNA replication. Unlike insulin, however, 20E was found to promote the expression of genes in GO categories describing a variety of developmental processes. These data represent the first description of the direct transcriptional response of the wing disc to either insulin or low doses of 20E.

2017-11-01 | GSE92933 | GEO

Drosophila melanogaster

Project description:Genome-wide Identification of Neuronal Activity-regulated Genes in Drosophila [KCl]

| PRJNA327582 | ENA

Drosophila melanogaster

Project description:Genome-wide Identification of Neuronal Activity-regulated Genes in Drosophila [Elav_TrpA1]

| PRJNA327583 | ENA

Drosophila melanogaster

Project description:Genome-wide Identification of Neuronal Activity-regulated Genes in Drosophila [Elav_ChR2]

| PRJNA327576 | ENA

Drosophila melanogaster

Project description:Genome-wide Identification of Neuronal Activity-regulated Genes in Drosophila [DA_PDF]

| PRJNA327578 | ENA

Drosophila melanogaster

Project description:Genome-wide Identification of Neuronal Activity-regulated Genes in Drosophila [Elav_TrpA1_ATAC]

| PRJNA327587 | ENA

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