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Single cell RNA-seq analysis of human dermal fibroblasts and human pluripotent stem cells

ABSTRACT: Single cell RNA-seq analysis of human dermal fibroblasts and human pluripotent stem cells

PROVIDER: PRJNA932226 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR23360415.fastq.gz	Fastqsanger.gz
	SRR23360416.fastq.gz	Fastqsanger.gz
	SRR23360417.fastq.gz	Fastqsanger.gz
	SRR23360418.fastq.gz	Fastqsanger.gz
	SRR23360419.fastq.gz	Fastqsanger.gz

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Pluripotent reprogramming arises from a unique subset of pre-disposed human dermal fibroblasts

Project description:Induced cellular reprogramming to the pluripotent state offers a novel stem cell source for autologous transplantation. While recent studies have explored the role of factors required for induced pluripotent stem cell (iPSC) induction, the cellular and molecular basis of reprogramming from human fibroblasts remains elusive. Here, we have identified a subset of human dermal-derived fibroblasts that shares hallmark molecular and epigenetic features with pluripotent cells. Functional studies demonstrate that these cells contribute to the majority of human iPSCs generated from dermal fibroblasts and are dependent on heterogeneous fibroblast microenvironment for reprogramming competency. Molecular characterization indicated these predisposed fibroblasts were unique to other dermal derived stem cells and possessed features of proliferative selfrenewal. Our study reveals human fibroblasts are not equivalently capable of cellular reprogramming, and suggests that reprogramming factors overcome commitment steps that allow predetermined dermal fibroblasts to establish stable pluripotent state.

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Single cell ATAC-seq analysis of human dermal fibroblasts and human pluripotent stem cells

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Project description:Expression profile of dermal fibroblasts reprogrammed to a pluripotent state Keywords: cell-type comparison

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Gene expression profiles of human dermal fibroblasts and human pluripotent stem cells analyzed by bulk RNA-seq

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Project description:Transcriptional analysis was performed on pre and post excision human induced pluripotent stem cells, the donor human dermal fibroblasts (HDFs) they were derived from and control human embryonic stem cells We isolated total RNA from pre and post excision human induced pluripotent stem cells, the donor human dermal fibroblasts (HDFs) they were derived from and control human embryonic stem cells and analyzed via Affymetrix microarray analysis.

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DNA Methylation profiling of human iNBSCs, iPSC-derived NBSCs and adult dermal fibroblasts

Project description:We describe a so far uncharacterized, embryonic and self-renewing Neural Plate Border Stem Cell (NBSC) population with the capacity to differentiate into central nervous and neural crest lineages. NBSCs can be obtained by neural transcription factor-mediated reprogramming (BRN2, SOX2, KLF4, and ZIC3) of human adult dermal fibroblasts and peripheral blood cells (induced Neural Plate Border Stem Cells, iNBSCs) or by directed differentiation from human induced pluripotent stem cells (NBSCs). Moreover, human (i)NBSCs share molecular and functional features with an endogenous NBSC population isolated from neural folds of E8.5 mouse embryos. Upon differentiation, iNBSCs give rise to either (1) radial glia-type stem cells, dopaminergic and serotonergic neurons, motoneurons, astrocytes, and oligodendrocytes or (2) cells from the neural crest lineage. Here we provide array-based methylation data of iNBSCs reprogrammed from adult dermal fibroblasts (ADF), iPSC-derived NBSCs and adult dermal fibroblasts. The data provided demonstrate robust changes in the methylation landscape after reprogramming of human adult dermal fibroblasts into iNBSCs.

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Project description:Comparison of gene expression profiles in three samples of normal human atrial cardiac fibroblasts, normal human ventricular cardiac fibroblasts, normal human dermal fibroblasts, human bone marrow-derived mesenchymal stem cells and human iPS cell-derived fibroblasts using microarray analysis.

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Project description:Dermal fibroblasts from human, stimulated with dsRNA (poly I:C) in a time course of 0, 2, 6 hours, profiled using the Smart-seq2 protocol

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