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Physiological and Molecular Characterization of an Boron-Resistant Saccharomyces cerevisiae Strain Obtained by Evolutionary Engineering

ABSTRACT: Physiological and Molecular Characterization of an Boron-Resistant Saccharomyces cerevisiae Strain Obtained by Evolutionary Engineering

PROVIDER: PRJNA932432 | ENA |

REPOSITORIES: ENA

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Physiological and Molecular Characterization of an Boron-Resistant Saccharomyces cerevisiae Strain Obtained by Evolutionary Engineering

Project description:One of the essential or beneficial micronutrient for plants and animals is boron that is an ultra-trace element. Although boron can inhibit the growth of Saccharomyces cerevisiae around 80 mM, it is also a growth supplement. However, little information is currently available regarding the molecular mechanisms and essentiality of boron. In this paper, the approach was to generate S. cerevisiae mutants with high boron resistance by using evolutionary engineering strategy that was previously applied successfully. Boron-resistant S. cerevisiae mutants were obtained and their phenotypic and physiological characteristics were determined. In order to identify the molecular mechanisms implicated in boron resistance, the whole transcriptomes and genome sequence analysis of wild type and one of the most resistant mutants were compared.

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Transcriptomic analysis of silver-resistant Saccharomyces cerevisiae mutant obtained by evolutionary engineering

Project description:Transcriptomic analysis of silver-resistant Saccharomyces cerevisiae mutant obtained by evolutionary engineering

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