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Homo sapiens strain:HCT-116,HT-29 | breed:cell

ABSTRACT: Homo sapiens strain:HCT-116,HT-29 | breed:cell Transcriptome or Gene expression

PROVIDER: PRJNA933002 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR23447973_1.fastq.gz	Fastqsanger.gz
	SRR23447973_2.fastq.gz	Fastqsanger.gz
	SRR23447974_1.fastq.gz	Fastqsanger.gz
	SRR23447974_2.fastq.gz	Fastqsanger.gz
	SRR23447975_1.fastq.gz	Fastqsanger.gz

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Barcode and Whole Exome Sequencing Data of Dabrafenib Resistant 3D-HT-29 and Irinotecan Resistant 3D-HCT-116 Spheroids

Project description:HT-29 and HCT-116 cells were barcoded using the CloneTracker lentiviral barcode library and then dabrafenib and irinotecan resistant derivatives of these cell lines were established, respectively.10 million barcoded HT-29 and HCT-116 cells were seeded equally onto poly-HEMA coated 4xT75 flask (DMSO Control, Replica A, B, C for each drug). After seeding, cells were allowed to form spheroids and barcoded 3D-HT-29 spheroids were treated with dabrafenib at increasing doses starting from IC50/10 dose until IC50/2 dose with monthly doubling of the dosing (16 weeks), and barcoded 3D-HCT-116 cells were treated with irinotecan at increasing doses starting from IC50/4 dose until IC50 dose with weekly doubling of the dosing (4 weeks). Following the end points of treatment for each cell line, DNA was isolated from harvested cell lines and barcode sequencing and whole exome sequencing were carried out.

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