Project description:In this study transcriptomic data of three life history stages of Orciraptor agilis was generated: 1) Gliding cells in absence of food ('gliding'), 2) Cells attached to the cell wall of its algal prey during perforation ('fattacking'), 3) Cells after acquisition of the algal plastid material ('digesting'). Furthermore, RNA-seq of the algal prey Mougeotia sp. was also performed. A de novo transcriptome assembly of the algal reads was performed in order to identify and substract algal reads of the Orciraptor samples by mapping the Orciraptor reads to the algal transcriptome. After this filtering step the remaining Orciraptor reads from all libraries were pooled for a de novo transcriptome assembly of Orciraptor agilis. This transcriptome was the basis for a comparative transcriptomic study in which transcript expression was compared between the three life history stages.

2022-06-13 | E-MTAB-11291 | biostudies-arrayexpress

RNAsequencing of Ligilactobacillus agilis grown on Inulin and Lactose

Project description:RNAsequening revolutionized the bacterial gene expression analysis. The objective of this study was to identify the genes involved in metabolism of Inulin in Ligilactobacillus agilis. We have obtained a list of genes upregulated in Ligilactobacillus agilis when it is grown in 1% Inulin

2020-10-27 | GSE160177 | GEO

Proteomic analysis of the papaverine degrading Arthrobacter sp.

Project description:In previous work in our group, shotgun genome sequencing of Arthrobacter sp. revealed potential new P450 monooxygenases and many other oxidoreductases with putative hydroxylation activity. A targeted approach to identify enzymes involved in the degradation of certain molecules is proteomic analysis. In the case of growth on certain substances, enzymes like P450s, which are responsible for the observed organism’s capabilities, might be overexpressed or initially induced.

2019-02-19 | PXD009566 | Pride

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