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Understanding differential heat tolerance of the threatened mountainous star coral Orbicella faveolata from inshore and offshore reef sites in the Florida Keys using gene network analysis

ABSTRACT: Understanding differential heat tolerance of the threatened mountainous star coral Orbicella faveolata from inshore and offshore reef sites in the Florida Keys using gene network analysis

PROVIDER: PRJNA933707 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR23409900_1.fastq.gz	Fastqsanger.gz
	SRR23409900_2.fastq.gz	Fastqsanger.gz
	SRR23409901_1.fastq.gz	Fastqsanger.gz
	SRR23409901_2.fastq.gz	Fastqsanger.gz
	SRR23409902_1.fastq.gz	Fastqsanger.gz

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Understanding differential heat tolerance of the threatened mountainous star coral Orbicella faveolata from inshore and offshore reef sites in the Florida Keys using gene network analysis

Project description:We subjected three inshore and four offshore genotypes of the coral Orbicella faveolata to 30, 31, 32, or 33ºC for 31 days and measured photochemical efficiency (Fv/Fm), the types and relative abundance of dinoflagellate endosymbionts, and gene expression of the host and symbiont. All inshore coral genotypes, regardless of symbiont type, were significantly more thermotolerant than offshore genotypes based on declines in Fv/Fm. The most heat-tolerant inshore genotype (In1) was dominated by Durusdinium trenchii; all other genotypes were Breviolum-dominated, suggesting local adaptation or acclimatization contributes to the heat tolerance of inshore genotypes. After 31 days of heat stress, all coral genotypes (except In2) had lost most of their Breviolum and became dominated by D. trenchii. Host genotype In1 presented unique expression patterns of genes involved in heat shock response, immunity, and protein degradation. There were few changes in the symbiont transcriptomes of inshore corals under heat stress, but significant changes in symbiont gene expression from the offshore colonies, including increases in ribosomal and photosynthetic proteins. These data show that the differential thermotolerance between inshore and offshore O. faveolata in the Florida Keys is associated with statistically significant differences in both host and symbiont gene expression that provide insights into the mechanisms underlying holobiont heat tolerance.

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Understanding differential heat tolerance of the threatened coral Orbicella faveolata from transplanted reef sites in the Florida Keys using gene network analysis

Project description:Twelve inshore and six offshore colonies were reciprocally transplanted during 1 year (July 2017- July 2018) at Florida Keys (location). After this period samples were collected from the field and brought to the Experimental Reef Laboratory facilities (RSMAS, Miami) to be acclimated to 30C during 7 days in six aquaria. Three aquaria were keep under initial conditions for the duration of the experiment (30C) and three aquaria had the temperature increased everyday during 7 days to a final temperature of 32C. A total of 56 samples were collected for RNAseq after 6 days of the temperature treatment and stored at -80C.

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Project description:Thirty-five Orbicella faveolata (scleractinian coral) proteins were labeled with iTRAQ technology and analyzed across five runs ("batches") of nano-liquid chromatography followed by mass spectrometry on a Q Exactive instrument. The samples represent a mix of corals that either were or were not reciprocally transplanted across shelves (inshore vs. offshore) in the Upper Florida Keys prior to a laboratory thermal challenge (tank) experiment. The goal of this project was to determine the molecular basis of thermotolerance in this common reef-builder as well as attempt to understand why inshore genotypes are typically more stress-tolerant than closely related offshore conspecifics.

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