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Effects of short distance transportation on physiological indexes, intestinal morphology, microbial community and transcriptome of jejunum in Weaned Piglets

ABSTRACT: Effects of short distance transportation on physiological indexes, intestinal morphology, microbial community and transcriptome of jejunum in Weaned Piglets

PROVIDER: PRJNA934118 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR23434981_1.fastq.gz	Fastqsanger.gz
	SRR23434981_2.fastq.gz	Fastqsanger.gz
	SRR23434982_1.fastq.gz	Fastqsanger.gz
	SRR23434982_2.fastq.gz	Fastqsanger.gz
	SRR23434983_1.fastq.gz	Fastqsanger.gz

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Effects of short distance transportation on physiological indexes, intestinal morphology, microbial community and transcriptome of jejunum in Weaned Piglets

Project description:To investigate the effect of short distance transport on jejunal tissueof weaned piglets, We then performed gene expression profiling analysis using data obtained from RNA-seq in jejunal tissues of weaned piglets after transport and without transport

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Project description:Transcriptional profiling of 25d old piglets comparing control untreated suckling jejunum with weaned piglets' jejunum. The goal was to gain new insight into the interaction between weaning and intestinal function.A keen interest is paid in deciphering expression changes of apoptosis or cell cycle control genes. The statistical analysis of gene ontology revealed that most of these altered genes are metabolic-related enzymes and regulators which may involved in the biological regulation, developmental process, and cellular process. Weaning also causes alterations in various immune response pathways. Results likely indicate that weaning induced cell cycle arrest, enhanced apoptosis, and inhibited cell proliferation. Two-condition experiment, suckling control piglets' jejunum vs. weaned piglets' jejunum. Biological replicates: 4 control replicates, 4 weaned replicates.

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Project description:Effects of short-distance transportation on physiological indexes, intestinal morphology, microbial community and the transcriptome of the jejunum in weaned piglets

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Transportation stress suppress reproductive performance in female mice and relevant genes detection

Project description:Animals in transport are exposed to both psychological and physical stressors, which affect performance and health. The objectives of this study were to investigate whether and how the transportation stress effect on female reproduction in mice, and detect differentially expressed genes association with stress response and reproductive capacity utilizing gene expression profiles. After transported mice by car to and from laboratory for a total period of 10 h, nearly 1000 km distance, measurements were carried out to explore effects of transportation stress on reproductive traits including puberty, estrous cycle, follicular number within ovaries, fertility and superovulation quality. Early life stress suffered mice before puberty promoted puberty onset, altered estrous cycle length and reduced large antral follicle number. Pubertal and adult mice exposure to transportation stress induced their pregnancy rate decline. Furthermore, adult stressed mice showed an obvious reduction in fertility with less litter size and litter weight in birth, which may cause reduced ovulation oocytes identified by superovulation. Assuming ovulated oocytes number as reproductive parameter, genes differently expressed between mice with extremely high and extremely low oocytes were compared within control and stress group separately.

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