Project description:The ami of this study was to investigate the possible biological effects of ELF-EMF by cDNA microarray. Keywords: ELF-EMF, cDNA microarray, keratinocyte, cytoskeleton
Project description:Use DNA microarray technology to discover transciprtional insights of HaCaT for continuous exposure to ELF-EMF We used a loop design in this study (details in the 'loop_design.tiff' supplementary file), cDNA microarray experiment consisted of ten RNA samples, including ELF-EMF exposed, UVB irradiated (positive control), and sham exposed samples.
Project description:Aberrant miRNA expression has been related to the development of human germ cell tumors, but little is known about effect of ELF-EMFs on miRNA expression. ELF-EMFs may epigenetically modify cells, which may account for the adverse effects of ELF-EMFs on the male reproductive system. To identify miRNAs that were differentially expressed between the sham and ELF-EMF exposure groups, we performed an Affymetrix microarray analysis to establish the miRNA expression profiles.
Project description:Aberrant miRNA expression has been related to the development of human germ cell tumors, but little is known about effect of ELF-EMFs on miRNA expression. ELF-EMFs may epigenetically modify cells, which may account for the adverse effects of ELF-EMFs on the male reproductive system. To identify miRNAs that were differentially expressed between the sham and ELF-EMF exposure groups, we performed an Affymetrix microarray analysis to establish the miRNA expression profiles. Mouse spermatocyte-derived GC-2 cells were intermittently exposed to a 50 Hz ELF-EMF for 72 h (5 min on/10 min off) at magnetic field intensities of 1 mT and 3 mT. miRNA expression was profiled using Affymetrix Mouse Genechip miRNA 3.0 arrays.
Project description:Graphene-based substrate has efficient neuronal differentiation of hMSCs. Stimulatory effects of graphene on hMSCs neurogenesis can be enhanced by ELF-EMF exposure and it is mediated by enhancement of cell adhesion accompanied by intracellular signal pathway. We used microarrays to assess up-regulated genes in human gene expression profiles during neurogenesis induced by ELF-EMF exposure on graphene. 3 groups (HMSCs grown in neuronal medium on glass, graphene, graphene under ELF-EMF exposure) were selected for RNA extraction and hybridization on Affymetrix microarrays. We sought to obtain total human gene expression. To that end, we hand-selected up-regulated gene involved in neurogenesis, extracellular matrix, and cell migration.
Project description:Graphene-based substrate has efficient neuronal differentiation of hMSCs. Stimulatory effects of graphene on hMSCs neurogenesis can be enhanced by ELF-EMF exposure and it is mediated by enhancement of cell adhesion accompanied by intracellular signal pathway. We used microarrays to assess up-regulated genes in human gene expression profiles during neurogenesis induced by ELF-EMF exposure on graphene.
Project description:The potential health hazard of exposures to electromagnetic fields (EMF) continues to cause much public concern. However, the biological and health effects of exposures to EMF remain controversial and their biophysical mechanisms are unclear. In the present study, we used Saccharomyces cerevisiae to identify genes responding to extremely low frequency magnetic fields (ELF-MF) and to radiofrequency (RF) EMF exposures. The expression of genes was analyzed by microarray screening and confirmed by real-time reverse transcription -polymerase chain reaction. In confirmation experiments, we found that there was no statistically significant change in three of the ELF-MF responsive candidate genes (P>0.05). On the other hand, out of the forty genes that responded to RF-EMF, the confirmation experiments found that only five were affected: structural maintenance of chromosomes 3-gene (SMC3), aquaporin 2 –gene (AQY2), halotolerance protein 9 –gene (HAL9), yet another kinase 1 -gene (YAK1) and one of unknown function gene (open reading frame: YJL171C) (P<0.05). Overall, this study has demonstrated that the yeast cells did not respond to 50 Hz ELF-MF and that the response to RF-EMF is limited to only five genes. The biological consequences of the observed gene expression changes induced by RF-EMF await further investigation. The yeast cells were exposed to 0.4 mT 50 Hz ELF-MF or 1800 MHz RF-EMF at a specific absorption rate of 3.5 W/kg for 6 hours.
