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Mature oligodendrocyte (OL) translatome changes at various times after moderate contusive spinal cord injury at the thoracic T9 level

ABSTRACT: Mature oligodendrocyte (OL) translatome changes at various times after moderate contusive spinal cord injury at the thoracic T9 level

PROVIDER: PRJNA934931 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR23458200.fastq.gz	Fastqsanger.gz
	SRR23458201.fastq.gz	Fastqsanger.gz
	SRR23458202.fastq.gz	Fastqsanger.gz
	SRR23458203.fastq.gz	Fastqsanger.gz
	SRR23458204.fastq.gz	Fastqsanger.gz

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Mature oligodendrocyte (OL) translatome changes at various times after moderate contusive spinal cord injury at the thoracic T9 level

Project description:OL translatome was determined in female RiboTag:Plp1-CreERT2 mice (C57Bl6 background) at 2, 10, and, 42 days after moderate contusive T9 SCI. Ribotag expression was induced by daily tamoxifen injections (1 mg i.p. over 8 days). Three weeks after completion of the induction protocol moderate contusive SCI was performed at T9 level (IH imactor, 50 kdyn). Uninjured mice (naive) were used as controls. At the time of surgery, animal age was 10-12 weeks. After euthanasia, RNA was isolated from a 5 mm spinal cord segment spanning the injury epicenter. OL tranlsatome was immunopurified using anti-HA antibody and protein A/G coupled magnetic beads following the Ribotag protocol. Total spinal cord RNA and OL translatome RNA samples were analyzed by RNASeq. Each biological sample was prepared by pooling material from two mice (3 biological samples representing 6 animals were used for each timepoint).

2023-12-06 | GSE225308 | GEO

Genome wide analysis of thoracic spinal cord at 5 days after T9 hemisection injury.

Project description:The goal of this study is to elucidate the influence of hemisection injury at thoracic spinal cord (T9) and epidural electrical spinal stimulation (L2-S1) on transcriptome of injured thoracic spinal cord. mRNA profiles of spinal cord at 5 days-post injury with or without epidural electrical spinal stimulation (L2-S1) and before injury were generated. Our study represents the detailed analysis of transcriptomes of injured spinal cord with biologic replicates, generated by RNA-seq technology.

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Project description:Genome wide analysis of thoracic spinal cord at 5 days after T9 hemisection injury.

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Project description:Vigna mungo strain:T9 | isolate:T9 Raw sequence reads

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Project description:Analysis of expression changes in prelabeled laser-microdissected thoracic propriospinal neurons at different times after low-thoracic spinal cord transection in adult rats.

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Caldicellulosiruptor owensensis OL strain:OL

Project description:Caldicellulosiruptor owensensis OL genome sequencing

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Genome wide analysis of the lower lumbar spinal cord at 4 days after T9 hemisection injury

Project description:The goal of this study is to elucidate the influence of hemisection injury at thoracic spinal cord (T9) on the transcriptome of the lower lumbar spinal cord at acute phase. mRNA profiles of spinal cord at 4 days-post injury and before injury were generated. 72 Differentially Expressed Genes (DEGs) were observed. Our study represents the detailed analysis of transcriptomes of spinal cord distal to the hemisected lesion at acute phase, with biologic replicates, generated by RNA-seq technology.

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Genome wide analysis of the upper lumbar spinal cord at 4 days after T9 hemisection injury

Project description:The goal of this study is to elucidate the influence of hemisection injury at thoracic spinal cord (T9) on the upper transcriptome of the upper lumbar spinal cord at acute phase. mRNA profiles of spinal cord at 4 days-post injury and before injury were generated. 72 Differentially Expressed Genes (DEGs) were observed. Our study represents the detailed analysis of transcriptomes of spinal cord distal to the hemisected lesion at acute phase, with biologic replicates, generated by RNA-seq technology.

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Project description:Analysis of expression changes in prelabeled laser-microdissected thoracic propriospinal neurons at different times after low-thoracic spinal cord transection in adult rats. Propriospinal neurons projecting to the lumbar enlargement were captured at various time points following no lesion or low thoracic spinal cord transection.

2010-05-05 | E-GEOD-20907 | biostudies-arrayexpress

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