Streptococcus pyogenes
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA93501 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:M1 and M6 serotypes, Wild-type vs. Mga- GAS
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Project description:JRS4 is our wild-type M6 serotype GAS and JRS519 is our Mga- M6 serotype GAS. SF370 is our wild-type M1 serotype GAS and KSM165L is our Mga- M1 serotype GAS. Table 1: Genomic Normalization Description: Step by Step instructions for the removal of outlier background data and any ORF data that did not meet threshold of 2SD above background average, using original data from gpr file provided in each of the sample files. Table 2: M1 Mga+ vs Mga- Description: Step by Step instructions for the removal of outlier data as compared across all possible data points for each ORF. Data points after genomic normalization are used with resultant averages and standard deviations for each ORF computed. Table 3: M6 Mga+ vs Mga- Description: Step by Step instructions for the removal of outlier data as compared across all possible data points for each ORF. Data points after genomic normalization are used with resultant averages and standard deviations for each ORF computed. Keywords: repeat sample
2005-04-06 | GSE1895 | GEO
Project description:JRS4 is our wild-type M6 serotype strain and JRS948 is the isogenic covR strain. Keywords: 2-channel
2005-12-19 | GSE3367 | GEO
Project description:GAS M49: wild type vs Δralp3 mutant
| PRJNA143401 | ENA
Project description:Two covR mutant derivatives of parental strain MGAS2221 were recovered from mice experimentally infected with MGAS2221 and shown to differ in terms of the number and concentration of secreted proteins. One of the covR mutant strains had a secretion phenotype identical to a covS mutant strain, while the other had a secretion phenotype identical to a constructed covR mutant strain. To further investigate the potential differences between the two covR mutant strains we performed expression microarray analysis. Single cultures of each of the four GAS strains tested were grown in THY broth to early exponential phase (O.D. 0.2). Two volumes of RNA protect were added, the samples incubated at room temperature for 5 minutes, and the bacteria collected through centrifugation. Total RNA was isolated via a mechaniscal disruption method, converted to cDNA, fragmented, labeled, and hybridized to our Affymetrix microarray. Estimates of gene expression were calculated using GCOS software v1.4. Data represent probes for serotype M1.
2009-06-05 | E-GEOD-16436 | biostudies-arrayexpress
Project description:JRS4 is our wild-type M6 serotype strain and JRS948 is the isogenic covR strain. Keywords: 2-channel Four separate RNA preparations from each strain (RNA #A-D) were hybridized to duplicate arrays. Reference RNA consisted of pooled RNA from each preparation, labelled with Cy5.
2005-12-19 | E-GEOD-3367 | biostudies-arrayexpress
Project description:S. pyogenes wild type vs. CvfA- mutant
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Project description:Transcriptional profile of Streptococcus pyogenes stk mutant strain JRS2516 vs its wild type parent strain MGAS2221
2011-08-01 | GSE28881 | GEO
Project description:In Streptococcus pyogenes, mutation of GidA results in avirulence despite the same growth rate as the wild type. To understand the basis of this effect, global transcription profiling was conducted. Keywords: Wild type vs. GidA mutant Streptococcus pyogenes
2008-01-10 | GSE9678 | GEO
Project description:Effect of CovR polymorphisms on GAS gene expression
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