Project description:Single-cell human genome analysis using whole-genome amplified product is hampered by allele bias during amplification. Using an oligonucleotide SNP array, we examined the nature of the allele bias and its effect on the chromosomal copy number analysis. Keywords: single cell, copy number analysis, whole genome amplification, brain
Project description:To evaluate the expression of genes associated with MYCN in NB, 10 tumors with MYCN amplification and 10 with normal MYCN copy number were subjected to oligonucleotide microarray using Agilent oligo microarray chips.
Project description:We examined the impact of chronic lung inflammation on the pulmonary transcriptional response to inhaled urban particles. Transcript levels were measured using high density microarrays in total RNA isolated from whole lungs of wildtype and TNF-α overexpressing mice exposed by inhalation to particulate matter and euthanized immediately or 24 h post-exposure. Experiment Overall Design: Transgenic SP-C/TNF-α mice and their wildtype littermates were exposed by inhalation to particulate matter (0, 42 mg/m3 EHC-93) for 4 h and euthanized immediately or 24 h post-exposure (n=5 per treatment per time point, 40 animals total). Agilent 22K oligonucleotide microarrays were used to examine transcript levels in whole lung RNA. Stratagene Universal Mouse RNA was used as a reference.
