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Single-cell Transcriptome analysis reveals cellular heterogeneity and highlights Fstl1-regulated alveolar myofibroblasts in mouse lung at birth

ABSTRACT: Single-cell Transcriptome analysis reveals cellular heterogeneity and highlights Fstl1-regulated alveolar myofibroblasts in mouse lung at birth

PROVIDER: PRJNA935749 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR23498151_1.fastq.gz	Fastqsanger.gz
	SRR23498151_2.fastq.gz	Fastqsanger.gz
	SRR23498152_1.fastq.gz	Fastqsanger.gz
	SRR23498152_2.fastq.gz	Fastqsanger.gz
	SRR23498153_1.fastq.gz	Fastqsanger.gz

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Single-cell Transcriptome analysis reveals cellular heterogeneity and highlights Fstl1-regulated alveolar myofibroblasts in mouse lung at birth

Project description:The matricellular protein follistatin-like 1 (FSTL1) regulates lung development and saccular formation. Here, we employed single-cell RNA sequencing (scRNA-seq) to construct a transcriptomic atlas of 22,774 individual cells in wild-type (WT) and Fstl1–/– lung (E18.5) samples and identified 27 cell subtypes. We reveal abnormal population sizes and gene expression profiles in diverse cell subtypes in Fstl1–/– lung samples. We identify Pdgfra and Tgfbi as genetic markers specifically expressed in postnatal myofibroblasts (MyoFBs). Fstl1 deletion decreases the number of MyoFB cells and downregulates their roles in ECM organization and muscle tissue/vasculature development, in part, through the TGF-β1/BMP4 signaling pathway. Our data provide a single-cell view of the cellular heterogeneity and the molecular mechanisms underlying abnormal saccular formation and atelectatic lungs in Fstl1–/– mice.

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Cancer-derived myofibroblasts vs. non-cancer derived myofibroblasts

Project description:All samples are conditioned media from primary cultured human gastric myofibroblasts, derived from either carcinoma tissue, or adjacent non cancerous tissue. In each experiment the cancer-derived myofibroblasts are compared against the non-cancer derived myofibroblasts from the same patient. All of the experiments were samples from Patient 1, except for 648, which is from Patient 2. SILAC labelling in each case is; 567 - Methionine COFRADIC; Cancer-derived = Heavy, Non-cancer = Light 575 - N-terminal COFRADIC; Cancer-derived = Heavy, Non-cancer = Light 646 - Cancer-derived = Light, Non-cancer = Heavy 647 - Non-cancer = Heavy, Non-cancer = Light (same cell line labelled twice to create a control experiment) 648 - Cancer-derived = Heavy, Non-cancer = Light

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