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A specific protective mechanism against chloroplast photo-reactive oxygen species in phosphate-starved rice plants

ABSTRACT: A specific protective mechanism against chloroplast photo-reactive oxygen species in phosphate-starved rice plants

PROVIDER: PRJNA935764 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR23499600_1.fastq.gz	Fastqsanger.gz
	SRR23499600_2.fastq.gz	Fastqsanger.gz
	SRR23499417_1.fastq.gz	Fastqsanger.gz
	SRR23499417_2.fastq.gz	Fastqsanger.gz
	SRR23499418_1.fastq.gz	Fastqsanger.gz

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A specific protective mechanism against chloroplast photo-reactive oxygen species in phosphate-starved rice plants [RNA-seq]

Project description:Phosphorus (Pi) starvation prevents a good match between light energy absorption and photosynthetic carbon metabolism. Photosynthetic electron-transport chain switches to use molecular oxygen as an electron carrier, generating photo-reactive oxygen species (photo-ROS) in chloroplast. In rice (Oryza sativa), DEEP GREEN PANICLE1 (DGP1) is robustly up-regulated in response to Pi-deficiency stress. DGP1 decreases the DNA-binding activities of the photosynthetic activators GLK1/2 on the genes involved in chlorophyll biosynthesis, light harvesting and electron transport. This Pi-starvation-induced mechanism dampens electron transport rates (ETRI and ETRII) and alleviates the electron-excessive stress in mesophyll cells. Meanwhile, DGP1 hijacks glycolytic enzymes GAPC1/2/3, redirecting glucose metabolism toward pentose phosphate pathway with superfluous NADPH production. Phenotypically, light irradiation induces O2– accumulation in Pi-starved WT leaves, but was observably accelerated in dgp1 mutant and impaired in GAPCsRNAi line and glk1glk2 double mutant. Interestingly, overexpression of DGP1 in rice caused hyposensitivity to the ROS-inducers (catechin and methyl viologen) and dgp1 mutant shows a similar inhibitory growth with the WT plants. We conclude that DGP1 gene serves as a specific antagonizer against Pi-starvation-induced photo-ROS, which integrates light-absorbing and anti-oxidative systems by orchestrating transcriptional and metabolic regulations, respectively.

2023-04-15 | GSE225472 | GEO

A specific protective mechanism against chloroplast photo-reactive oxygen species in phosphate-starved rice plants [ChIP-seq]

2023-04-15 | GSE225476 | GEO

A specific protective mechanism against chloroplast photo-reactive oxygen species in phosphate-starved rice plants [RNA-seq]

Project description:A specific protective mechanism against chloroplast photo-reactive oxygen species in phosphate-starved rice plants [RNA-seq]

| PRJNA935766 | ENA

A specific protective mechanism against chloroplast photo-reactive oxygen species in phosphate-starved rice plants [ChIP-seq]

Project description:A specific protective mechanism against chloroplast photo-reactive oxygen species in phosphate-starved rice plants [ChIP-seq]

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A specific protective mechanism against chloroplast photo-reactive oxygen species in phosphate-starved rice plants

Project description:This SuperSeries is composed of the SubSeries listed below.

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Project description:We established a transcriptome profiling method coupled with photo-isolation chemistry (PIC) that allows the determination of expression profiles specifically from photo-irradiated regions of interest.

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Project description:Cross-linking mass spectrometry (XL-MS) is an emerging technique to obtain structural information of biomacromolecules and their complexes in cells and in vitro. In particular, certain photo-reactive amino acids (pA) such as photo-leucine (pLeu) and photo-methionine can provide unique short-distance information on structural core regions of proteins. Here, we present a protocol for high-yield incorporation of pLeu in proteins recombinantly expressed in Escherichia coli. The protein is expressed at high cell densities, which reduces the required amount of the photo-reactive amino acid by a factor of 10 compared to standard protocols, while maintaining high incorporation rates. For the two chaperones Trigger Factor and SecB, up to 3 mg of photo-leucine labeled protein were thus obtained from 100 ml of cell culture, with label incorporation rates of up to 34%. For Trigger factor, UV-induced cross-linking lead to the identification of 12 cross-links that are in agreement with published three-dimensional structures. The accessibility of milligram amounts of photo-leucine-labeled proteins at low costs will be highly useful to address structural biology questions.

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