Bos taurus
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA93645 | ENA |
REPOSITORIES: ENA
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Project description:Expression data from early preimplantation Bovine embryos
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Project description:Affymetrix Human GeneChips are used to profile gene expression of bovine tissues and embryos to identify uniquely expressed genes in bovine in-vitro fertilized embryos by comparing with seven bovine adult tissues through gene clustering
2007-07-19 | E-GEOD-1638 | biostudies-arrayexpress
Project description:To generate expression profiles from single embryos, a protocol for linear amplification of RNA was developed on the basis of Baugh et al. (15). Linear amplification enabled production of sufficient amounts of aRNA for the microarray experiments and has been shown to reliably amplify the initial mRNA population (15, 18-20). Approximately 5 ng of total RNA can be isolated from a blastocyst-stage embryo (21); therefore, it was necessary to carry out multiple rounds of amplification. To test the reproducibility and fidelity of the amplification procedure, a validation experiment was conducted using RNA from the kidney tissue of a newborn calf. Two replicates of the bovine kidney RNA were amplified and hybridized to the microarray. Comparisons were made between aRNA (rounds 1, 2, and 3) and unamplified RNA. Keywords: cDNA microarray
2005-11-30 | GSE3569 | GEO
Project description:Gene expression profiles of in vitro fertilized bovine embryos in comparison to those of bovine adult tissues
| PRJNA90805 | ENA
Project description:The process of early development of mammals is subtly and accurately controlled by the regulation networks of embryo cells. Time course expression data measured at different stages during early embryo development process can give us valuable information by revealing the dynamic expression patterns of genes in genome wide scale. In this study, bovine embryo expression data were generated at oocyte, one cell stage, two cell stage, four cell stage, eight cell stage, sixteen cell stage, morula, and blastocyst; Human embryo expression data were generated at one cell stage, two cell stage, four cell stage, eight cell stage, morula, and blastocyst; Mouse embryo expression data were generated at one cell stage, two cell stage, four cell stage, eight cell stage, morula, and blastocyst. Experiment Overall Design: Bovine, Human, and Mouse embryos were harvested at successive stage from oocyte to blastocyste. Total RNAs were extracted, amplified and hybridized onto Affymetrix microarrays.
2009-10-02 | E-GEOD-18290 | biostudies-arrayexpress
Project description:Influence Of Insulin On Oocyte Maturation In Invitro Produced Bovine Embryos
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Project description:Metabolic Stress Induces Modifications in the Epigenetic Program of Preimplantation Bovine Embryos
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Project description:Effect of maternal methionine supplementation on the transcriptome of bovine preimplantation embryos
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