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A single-cell RNA sequencing atlas of circulating leukocytes from healthy and osteosarcoma affected dogs

ABSTRACT: A single-cell RNA sequencing atlas of circulating leukocytes from healthy and osteosarcoma affected dogs

PROVIDER: PRJNA936588 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR23525318_1.fastq.gz	Fastqsanger.gz
	SRR23525318_2.fastq.gz	Fastqsanger.gz
	SRR23525319_1.fastq.gz	Fastqsanger.gz
	SRR23525319_2.fastq.gz	Fastqsanger.gz
	SRR23525320_1.fastq.gz	Fastqsanger.gz

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A single-cell RNA sequencing atlas of circulating leukocytes from healthy and osteosarcoma affected dogs

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Project description:Osteosarcoma in dogs is a spontaneously occurring disease with a global tumor gene expression signature indistinguishable from human pediatric tumors and clinical progression is remarkably similar. Unlike human OS, canine OS is a highly heritable disease with some large and giant dog breeds at >10x increased risk. We did a genome wide association study of osteosarcoma using the Illumina CanineHD genotyping array in three breeds: greyhound (mortality from OS = 26%), rottweiler (17%) and Irish wolfhound (IWH, 21%) and identified 33 inherited risk loci explaining 55 to 85% of phenotype variance in each breed. Data created for a genome-wide association studio of heritable osteosarcoma risk factors in dogs, including 267 racing greyhounds (153 affected (A) + 114 unaffected (U)), 135 rottweilers (80 A + 55 U), 141 IWH (76 A + 65 U) and 19 AKC greyhounds using the Illumina CanineHD array (~170,000 SNPs genomewide)

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