Project description:This study aims to fully characterize the changes taht undergoes hepatic transcriptome due to dietically induced steatosis in commercial crossbreed pigs
Project description:We investigated the effect of feeding mice a Total Western Diet formulated using the 50th percentile daily intake levels for macro and micronutrients from the National Health and Nutrition Examination Survey (NHANES) with 0, 2, 5, or 10% added raw potato starch on the cecal microbiome (16S) and cecum, proximal and distal colon gene expression by RNASeq analysis.
Project description:Today, swine is regarded as promising biomedical model, however, its gastrointestinal microbiome dynamics have been less investigated than that of humans or murine models . The aim of this study was to establish a high-throughput multi-omics pipeline to investigate the healthy fecal microbiome of swine and its temporal dynamics as basis for future infection studies. To this end, a homogenization protocol based on deep-frozen feces followed by integrated sample preparation for different meta-omics analyses was developed. Subsequent data integration linked microbiome composition with function, i.e. expressed proteins and secreted metabolites.
Project description:Muscle development is associated with a modification of mass, composition and function that in productive animals are considered responsible of meat characteristics. In this study, SAGE was employed to describe gene expression in pigs of two commercial lines at the 3 and 9 months og age. The aim was to obtained a full gene expression profile that would assist in identifying genes differentially expressed in the muscle Longissimum dorsi during normal growth. The pattern of expression between lines and ages will help to elucidate the metabolic pathways modulated by these genes during muscle development. Keywords: SAGE, muscle, swine, age comparison, swine comparison
Project description:As a mild, highly contagious, respiratory disease, swine influenza always damages the innate immune systems, and increases susceptibility to secondary infections which results in considerable morbidity and mortality in pigs. Nevertheless, the systematical host response of pigs to swine influenza virus infection remains largely unknown. To explore these, a time-course gene expression profiling was performed to detect comprehensive analysis of the global host response induced by H1N1 swine influenza virus in pigs.
